1. Volume 115, Issue 3, Pages 618-627 (September 1998)
Nitric oxide modulates T-lymphocyte migration in Peyer's patches and villous submucosa of rat small intestine 
Ryota Hokari*, Soichiro Miura‡, Hitoshi Fujimori*, Yoshikazu Tsuzuki*, Takeharu Shigematsu*, Hajime Higuchi*, Hiroyuki Kimura*, Iwao Kurose*, Hiroshi Serizawa*, Makoto Suematsu§, Hideo Yagita∥, D.Neil Granger¶, Hiromasa Ishii* 
Gastroenterology 
Volume 115, Issue 3, Pages (September 1998)
DOI: /S (98)
Copyright © 1998 American Gastroenterological Association Terms and Conditions

2. Fig. 1
Effect of L-NAME administration on percentage of free-flowing and rolling lymphocytes in (A) postcapillary venules of rat Peyer's patches and (B) submucosal venules of villi within 10 minutes after infusion of CFSE-labeled T lymphocytes. Also shown is the attenuating effect by L-arginine. The lymphocytes were counted for 1 minute and represented as the percentage of all lymphocytes entered into the observation field. D-NAME or L-NAME (100 μmol/kg) was administered from the jugular vein 30 minutes before lymphocyte infusion. L-Arginine (500 μmol/kg) was infused from the jugular vein 10 minutes before L-NAME injection. *P < 0.05 compared with D-NAME. #P < 0.05 compared with L-NAME. Values are mean ± SEM from 6 animals. ▨, D-NAME; ■, L-NAME; ▩, L-NAME + L-arginine.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

3. Fig. 1
Effect of L-NAME administration on percentage of free-flowing and rolling lymphocytes in (A) postcapillary venules of rat Peyer's patches and (B) submucosal venules of villi within 10 minutes after infusion of CFSE-labeled T lymphocytes. Also shown is the attenuating effect by L-arginine. The lymphocytes were counted for 1 minute and represented as the percentage of all lymphocytes entered into the observation field. D-NAME or L-NAME (100 μmol/kg) was administered from the jugular vein 30 minutes before lymphocyte infusion. L-Arginine (500 μmol/kg) was infused from the jugular vein 10 minutes before L-NAME injection. *P < 0.05 compared with D-NAME. #P < 0.05 compared with L-NAME. Values are mean ± SEM from 6 animals. ▨, D-NAME; ■, L-NAME; ▩, L-NAME + L-arginine.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

4. Fig. 2
Effect of L-NAME administration on the time course of lymphocyte sticking in (A) postcapillary venules of rat Peyer's patches and (B) submucosal venules of villi within 10 minutes after infusion of CFSE-labeled T lymphocytes. Also shown is the attenuating effect of L-arginine. The lymphocytes located both inside and along venules were counted in the 1-mm2 observation field. D- or L-NAME (100 μmol/kg) was administered from the jugular vein 30 minutes before lymphocyte infusion. L-Arginine (500 μmol/kg) was infused from the jugular vein 10 minutes before L-NAME injection. *P < 0.05 compared with D-NAME. #P < 0.05 compared with L-NAME. Values are mean ± SEM from 6 animals. □, D-NAME; ●, L-NAME; ■, L-NAME + L-arginine.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

5. Fig. 2
Effect of L-NAME administration on the time course of lymphocyte sticking in (A) postcapillary venules of rat Peyer's patches and (B) submucosal venules of villi within 10 minutes after infusion of CFSE-labeled T lymphocytes. Also shown is the attenuating effect of L-arginine. The lymphocytes located both inside and along venules were counted in the 1-mm2 observation field. D- or L-NAME (100 μmol/kg) was administered from the jugular vein 30 minutes before lymphocyte infusion. L-Arginine (500 μmol/kg) was infused from the jugular vein 10 minutes before L-NAME injection. *P < 0.05 compared with D-NAME. #P < 0.05 compared with L-NAME. Values are mean ± SEM from 6 animals. □, D-NAME; ●, L-NAME; ■, L-NAME + L-arginine.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

6. Fig. 3
Representative image of the distribution of migrated CFSE-labeled T lymphocytes in Peyer's patches at about 15 minutes (A and C) and about 60 minutes (B and D) after infusion in (A and B) D-NAME–treated and (C and D) L-NAME–treated rats. Administration of L-NAME significantly enhanced the number of sticking lymphocytes at (C) 15 minutes compared with (A) D-NAME–treated control. L-NAME treatment also significantly enhanced the transmigration of T lymphocytes through the wall of postcapillary venules compared with controls. At 60 minutes, most lymphocytes were observed inside the PCVs or just outside of the venules after extravasation in (C) D-NAME–treated rats, whereas a significant number of lymphocytes had migrated into the interstitium of Peyer's patches for various distances in (D) L-NAME–treated rats. Bar = 100 μm.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

7. Fig. 4
Effect of L-NAME administration on the time course of the flux of CFSE-labeled T lymphocytes migrating into the microlymphatics of Peyer's patches. Also shown is the attenuating effect of L-arginine. Values are mean + SEM of six animal experiments. The appearance of lymphocytes in the designated area in lymphatics was counted over a 10-minute period. *P < 0.05 compared with the values of D-NAME. *P < 0.05 vs. L-NAME. □, D-NAME; ●, L-NAME; ■, L-NAME + L-arginine.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

8. Fig. 5
Effect of MAb against β2-integrin (CL26; F(ab')2 fragments), α4-integrin (MRα4-1; F(ab')2 fragments), L-selectin (HRL-3; IgG), and P-selectin (PB1.3; IgG) on L-NAME–induced T-lymphocyte rolling at 10 minutes in PCVs of (A) Peyer's patches and (B) submucosal venules of villi. Values are expressed as the percentage of rolling lymphocytes in the L-NAME–treated animals. Lymphocytes were preincubated with CL26, MRα4-1, and HRL-3 for 20 minutes before infusion. In case of P-selectin, PB1.3 was infused to rats 30 minutes before L-NAME treatment. #P < 0.05 compared with L-NAME. Values are mean ± SEM from 6 animals. ■, L-NAME alone; ●, +CL26; ▨, +MRα4-1; ▩, +HRL-3; □, +PB1.3; ▤, +MRα4-1 and PB1.3.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

9. Fig. 5
Effect of MAb against β2-integrin (CL26; F(ab')2 fragments), α4-integrin (MRα4-1; F(ab')2 fragments), L-selectin (HRL-3; IgG), and P-selectin (PB1.3; IgG) on L-NAME–induced T-lymphocyte rolling at 10 minutes in PCVs of (A) Peyer's patches and (B) submucosal venules of villi. Values are expressed as the percentage of rolling lymphocytes in the L-NAME–treated animals. Lymphocytes were preincubated with CL26, MRα4-1, and HRL-3 for 20 minutes before infusion. In case of P-selectin, PB1.3 was infused to rats 30 minutes before L-NAME treatment. #P < 0.05 compared with L-NAME. Values are mean ± SEM from 6 animals. ■, L-NAME alone; ●, +CL26; ▨, +MRα4-1; ▩, +HRL-3; □, +PB1.3; ▤, +MRα4-1 and PB1.3.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

10. Fig. 6
Effect of MAb against β2-integrin (CL26; F(ab')2 fragments), α4-integrin (MRα4-1; F(ab')2 fragments). L-selectin (HRL-3; IgG) and P-selectin (PB1.3; IgG) on L-NAME–induced lymphocyte sticking at 30 minutes after the infusion of T lymphocytes in postcapillary venules of (A) Peyer's patches and (B) submucosal venules of villi. Values are expressed as the percentage of sticking lymphocytes in the L-NAME–treated animals. #P < 0.05 compared with L-NAME. Values are mean ± SEM from 6 animals. ■, L-NAME alone; ●, +CL26; ▨, +MRα4-1; ▩, +HRL-3; □, +PB1.3, ▤, +MRα4-1 and PB1.3.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

11. Fig. 6
Effect of MAb against β2-integrin (CL26; F(ab')2 fragments), α4-integrin (MRα4-1; F(ab')2 fragments). L-selectin (HRL-3; IgG) and P-selectin (PB1.3; IgG) on L-NAME–induced lymphocyte sticking at 30 minutes after the infusion of T lymphocytes in postcapillary venules of (A) Peyer's patches and (B) submucosal venules of villi. Values are expressed as the percentage of sticking lymphocytes in the L-NAME–treated animals. #P < 0.05 compared with L-NAME. Values are mean ± SEM from 6 animals. ■, L-NAME alone; ●, +CL26; ▨, +MRα4-1; ▩, +HRL-3; □, +PB1.3, ▤, +MRα4-1 and PB1.3.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

12. Fig. 7
Effect of aminoguanidine administration on percentage of free-flowing and rolling lymphocytes at 10 minutes and number of sticking lymphocytes at 30 minutes after infusion of CFSE-labeled T lymphocytes in postcapillary venules of (A) rat Peyer's patches and (B) submucosal venules of villi. Values are mean ± SEM from 6 animals. □, Control; ▨, aminoguanidine.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

13. Fig. 7
Effect of aminoguanidine administration on percentage of free-flowing and rolling lymphocytes at 10 minutes and number of sticking lymphocytes at 30 minutes after infusion of CFSE-labeled T lymphocytes in postcapillary venules of (A) rat Peyer's patches and (B) submucosal venules of villi. Values are mean ± SEM from 6 animals. □, Control; ▨, aminoguanidine.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions