Presentation is loading. Please wait.

Presentation is loading. Please wait.

A Multicenter Blinded Study Evaluating EGFR and KRAS Mutation Testing Methods in the Clinical Non–Small Cell Lung Cancer Setting—IFCT/ERMETIC2 Project.

Published byJean-Marc Rondeau Modified over 5 years ago

Similar presentations

Presentation on theme: "A Multicenter Blinded Study Evaluating EGFR and KRAS Mutation Testing Methods in the Clinical Non–Small Cell Lung Cancer Setting—IFCT/ERMETIC2 Project."— Presentation transcript:

1 A Multicenter Blinded Study Evaluating EGFR and KRAS Mutation Testing Methods in the Clinical Non–Small Cell Lung Cancer Setting—IFCT/ERMETIC2 Project Part 1  Michèle Beau-Faller, Hélène Blons, Caroline Domerg, Dorota Gajda, Nicolas Richard, Fabienne Escande, Jérôme Solassol, Marc G. Denis, Anne Cayre, Isabelle Nanni-Metellus, Sylviane Olschwang, Sarab Lizard, Fabienne Piard, Jean-Luc Pretet, Florence de Fraipont, Ivan Bièche, Patricia de Cremoux, Isabelle Rouquette, Pierre-Paul Bringuier, Jean Mosser, Michèle Legrain, Anne-Claire Voegeli, Patrick Saulnier, Franck Morin, Jean-Pierre Pignon, Gérard Zalcman, Jacques Cadranel  The Journal of Molecular Diagnostics  Volume 16, Issue 1, Pages (January 2014) DOI: /j.jmoldx Copyright © 2014 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

2 Figure 1 Percentage of centers detecting the mutation in diluted cell line DNA. A: EGFR exon 18, p.G719S (SW48). B: EGFR exon 19, p.E746_750del (H1650). C: EGFR exon 20, p.T790M (H1975). D: EGFR exon 21, p.L858R (H1975). E: KRAS exon 2, p.G12C (H358). MASO, multiplex allele-specific oligonucleotide. The Journal of Molecular Diagnostics  , 45-55DOI: ( /j.jmoldx ) Copyright © 2014 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

3 Figure 2 Box plot of the percentage of nonamplificable (NA) NSCLC paraffin-embedded samples recovered by alternative techniques of the 74 tested. The bottom and top of the box are the 25th and 75th percentiles (the lower and upper quartiles), respectively, and the horizontal line near the middle of the box is the 50th percentile (the median). The error bars are defined by the lowest datum still within 1.5 interquartile range (IQR) of the lower quartile and the highest datum still within 1.5 IQR of the upper quartile. The number of experiments in which NA recovery rates were computed is indicated in parentheses for each alternative technique. One experiment corresponds to a set of amplicons analyzed for a given exon and a given technique in one center. FA, fragment analysis; HRM, high resolution melting; PYRO, pyrosequencing; TT, targeted techniques. The Journal of Molecular Diagnostics  , 45-55DOI: ( /j.jmoldx ) Copyright © 2014 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

4 Figure 3 Box plots of kappa values by exon. The number of kappas computed, one for each experiment defined by the combination of exon, technique, and center, is indicated in parentheses for each alternative technique. Kappa SQC, between direct sequencing and alternative molecular techniques (P < 0.001); Kappa SYNTH, between alternative molecular techniques and a reference based on all alternative techniques (P = 0.04). e18, EGFR exon 18; e19, EGFR exon 19; e20, EGFR exon 20; e21, EGFR exon 21. See the legend to Figure 2 for a description of the elements of the box and whisker plot. The Journal of Molecular Diagnostics  , 45-55DOI: ( /j.jmoldx ) Copyright © 2014 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

5 Figure 4 Box plots of Kappa SYNTH values by groups of alternative techniques. The number of kappas is indicated in parentheses for each alternative technique. Results between alternative techniques were not significantly different (P = 0.3). TT, targeted techniques; FA, fragment analysis; HRM, high resolution melting; PYRO, pyrosequencing. See the legend to Figure 2 for a description of the elements of the box and whisker plot. The Journal of Molecular Diagnostics  , 45-55DOI: ( /j.jmoldx ) Copyright © 2014 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Download ppt "A Multicenter Blinded Study Evaluating EGFR and KRAS Mutation Testing Methods in the Clinical Non–Small Cell Lung Cancer Setting—IFCT/ERMETIC2 Project."

Similar presentations

Quartiles & Extremes (displayed in a Box-and-Whisker Plot) Lower Extreme Lower Quartile Median Upper Quartile Upper Extreme Back.

Quartiles & Extremes (displayed in a Box-and-Whisker Plot) Lower Extreme Lower Quartile Median Upper Quartile Upper Extreme Back.
Clinical Laboratory Analysis of Immunoglobulin Heavy Chain Variable Region Genes for Chronic Lymphocytic Leukemia Prognosis  Philippe Szankasi, David.

Clinical Laboratory Analysis of Immunoglobulin Heavy Chain Variable Region Genes for Chronic Lymphocytic Leukemia Prognosis  Philippe Szankasi, David.
Impact of Systematic EGFR and KRAS Mutation Evaluation on Progression-Free Survival and Overall Survival in Patients with Advanced Non–Small-Cell Lung.

Impact of Systematic EGFR and KRAS Mutation Evaluation on Progression-Free Survival and Overall Survival in Patients with Advanced Non–Small-Cell Lung.
MET Gene Copy Number in Non-small Cell Lung Cancer: Molecular Analysis in a Targeted Tyrosine Kinase Inhibitor Naïve Cohort  Michèle Beau-Faller, MD,

MET Gene Copy Number in Non-small Cell Lung Cancer: Molecular Analysis in a Targeted Tyrosine Kinase Inhibitor Naïve Cohort  Michèle Beau-Faller, MD,
Molecular Analysis of the Breast Cancer Genes BRCA1 and BRCA2 Using Amplicon- Based Massive Parallel Pyrosequencing  Geneviève Michils, Silke Hollants,

Molecular Analysis of the Breast Cancer Genes BRCA1 and BRCA2 Using Amplicon- Based Massive Parallel Pyrosequencing  Geneviève Michils, Silke Hollants,
A Multicenter Blinded Study Evaluating EGFR and KRAS Mutation Testing Methods in the Clinical Non–Small Cell Lung Cancer Setting—IFCT/ERMETIC2 Project.

A Multicenter Blinded Study Evaluating EGFR and KRAS Mutation Testing Methods in the Clinical Non–Small Cell Lung Cancer Setting—IFCT/ERMETIC2 Project.
Detection of Exon 12 Mutations in the JAK2 Gene

Detection of Exon 12 Mutations in the JAK2 Gene
The Use of COLD-PCR and High-Resolution Melting Analysis Improves the Limit of Detection of KRAS and BRAF Mutations in Colorectal Cancer  Irene Mancini,

The Use of COLD-PCR and High-Resolution Melting Analysis Improves the Limit of Detection of KRAS and BRAF Mutations in Colorectal Cancer  Irene Mancini,
Comparison of Clinical Targeted Next-Generation Sequence Data from Formalin-Fixed and Fresh-Frozen Tissue Specimens  David H. Spencer, Jennifer K. Sehn,

Comparison of Clinical Targeted Next-Generation Sequence Data from Formalin-Fixed and Fresh-Frozen Tissue Specimens  David H. Spencer, Jennifer K. Sehn,
Mutant Enrichment with 3′-Modified Oligonucleotides

Mutant Enrichment with 3′-Modified Oligonucleotides
Simultaneous Detection of Clinically Relevant Mutations and Amplifications for Routine Cancer Pathology  Marlous Hoogstraat, John W.J. Hinrichs, Nicolle.

Simultaneous Detection of Clinically Relevant Mutations and Amplifications for Routine Cancer Pathology  Marlous Hoogstraat, John W.J. Hinrichs, Nicolle.
A Strategy to Find Suitable Reference Genes for miRNA Quantitative PCR Analysis and Its Application to Cervical Specimens  Iris Babion, Barbara C. Snoek,

A Strategy to Find Suitable Reference Genes for miRNA Quantitative PCR Analysis and Its Application to Cervical Specimens  Iris Babion, Barbara C. Snoek,
Haley J. Abel, Hussam Al-Kateb, Catherine E. Cottrell, Andrew J

Haley J. Abel, Hussam Al-Kateb, Catherine E. Cottrell, Andrew J
Single-Color Digital PCR Provides High-Performance Detection of Cancer Mutations from Circulating DNA  Christina Wood-Bouwens, Billy T. Lau, Christine.

Single-Color Digital PCR Provides High-Performance Detection of Cancer Mutations from Circulating DNA  Christina Wood-Bouwens, Billy T. Lau, Christine.
Laboratory Guidelines for Detection, Interpretation, and Reporting of Maternal Cell Contamination in Prenatal Analyses  Narasimhan Nagan, Nicole E. Faulkner,

Laboratory Guidelines for Detection, Interpretation, and Reporting of Maternal Cell Contamination in Prenatal Analyses  Narasimhan Nagan, Nicole E. Faulkner,
False Positives in Multiplex PCR-Based Next-Generation Sequencing Have Unique Signatures  Chad M. McCall, Stacy Mosier, Michele Thiess, Marija Debeljak,

False Positives in Multiplex PCR-Based Next-Generation Sequencing Have Unique Signatures  Chad M. McCall, Stacy Mosier, Michele Thiess, Marija Debeljak,
Improving Mutation Screening in Patients with Colorectal Cancer Predisposition Using Next-Generation Sequencing  Jean-Marc Rey, Vincent Ducros, Pascal.

Improving Mutation Screening in Patients with Colorectal Cancer Predisposition Using Next-Generation Sequencing  Jean-Marc Rey, Vincent Ducros, Pascal.
Multiplex Preamplification of Serum DNA to Facilitate Reliable Detection of Extremely Rare Cancer Mutations in Circulating DNA by Digital PCR  Jennifer.

Multiplex Preamplification of Serum DNA to Facilitate Reliable Detection of Extremely Rare Cancer Mutations in Circulating DNA by Digital PCR  Jennifer.
A Multiplex SNaPshot Assay as a Rapid Method for Detecting KRAS and BRAF Mutations in Advanced Colorectal Cancers  Sandrine Magnin, Erika Viel, Alice.

A Multiplex SNaPshot Assay as a Rapid Method for Detecting KRAS and BRAF Mutations in Advanced Colorectal Cancers  Sandrine Magnin, Erika Viel, Alice.
Detection of CALR and MPL Mutations in Low Allelic Burden JAK2 V617F Essential Thrombocythemia  Fabrice Usseglio, Nathalie Beaufils, Anne Calleja, Sophie.

Detection of CALR and MPL Mutations in Low Allelic Burden JAK2 V617F Essential Thrombocythemia  Fabrice Usseglio, Nathalie Beaufils, Anne Calleja, Sophie.

Similar presentations

Ads by Google