3. Spectral Karyotyping

4. Immune system

10. Complement System

11. Antibody (Immunoglobulin)

12. Antibody diversity

13. B cell maturation

14. Ig G

15. Ig E

16. Ig A

17. Boost and memory

22. Monoclonal antibody
HGPRTase: hypoxanthine-guanine phosphoribosyltransferase
HAT media: Hypoxanthine, aminopterin, thymine medium

23. Humanized antibody
CDRs: Complementarity Determining Region

24. Magic bullets
For transplantation rejection anti-IL-2R (p55alpha, CD25, Tac)
Zenapax (Daclizumab): 90% human and 10% murine
Simulect (Basiliximab): 70% human and 30% murine
For Breast cancer anti HER2/neu R (erbB2R)
Herceptin (Trastuzumab)
For Non-Hdgkin’s Lymphoma anti CD20
Rituxan / MabThera (Rituximab)
For ectoderm origin tumor anti EGFR
Nimotuzumab
Vectibix (panitumumab: IgG2)
Erbitux (cetuximab: IgG1)
For AMD anti VEGF
Lucentis (Ranibizumab)
Avastin (Bevacizumab)
Macugen (pegaptanib)
For autoimmune disease anti TNFalpha
Humira (Adalimumab)
Remicade (Infliximab)
anti IL-1R
Anakinra
soluble TNFR
Enbrel (Etanercept)
For asthma anti Ig E
Xolair (Omalizumab)

26. Take home message
Function of immunoglobulin
Function of T cell