1. An Investigation of Oil on Coral Beaching
Nathalie
Betty
Cristina
Saskia
Shannon

2. Group 3 Control Experiments
In these experiments results were taken under the control conditions for the tank. These results will be used to compare to the results obtained in the experimental tank.
Temperature: 240C
Salinity: 34 ppt
Experiments performed: zoozanthellae count, protein assay, and chlorophyll extraction.

3. Zoozanthellae Count avg of 5 counts of .004m^3 =126.2 zoox
.004mm^3  x   1ml            = 4 x 10^-6ml
                  1000mm^3
126.2 zoox  =3.16 x 10^7 zoox/ml
4 x 10^-6ml
2ml(total homogenate volume) x 3.16 x 10^7 zoox/ml  = 6.31 x 10^7 zoox
zoox density:    6.31x 10^7 zoox= 7.01 x 10^8 zoox/g wet wt
                    
.09 g wet wt

4. Protein Assay Protein (mg/mL) ABS 0.25 0.443 0.5 0.721 0.75 1.0785 1
1.405
1.25
1.59
Unknown
0.806
y= X
y= .806
By substitution x = .544 mg/mL of protein

5. Chlorophyll Extraction
Wavelength
Chlorophyll
665
0.022
647
0.011
630
0.021
Ca= (11.85x.022)-(1.54x.011)-(.08x.021)
=.24205
Mg Chl a/m3 = x 9 / 1x1.6 = 1.351

6. Water Accommodated Fraction (WAF) Production and Analysis
In order to know the concentration of oil to add to tank we first need to how much oil dissolves in water (WAF).
Prepared 36 g of Na and 900 mL of tap water.
The oil (Quaker State) was then added at a ratio of 9:1 (900ml seawater:100ml oil). The seawater and oil solution was mixed using a magnetic stirrer for 20 hours and left to settle for the next week to use in each experiment.

8. Today Today we will calculate the WAF.
We will also try to begin the experiment by adding a estimated amount of oil and seeing what happens. By taking the 3 test counts we can determine if the added amount of oil was too much or too little.