1. Phospho IRF-7 (Ser 477/Ser479)
assay
Cellular kinase MTO

2. Contents General introduction Signaling pathway Assay format
Product specifications
Assay performances
Ordering information
Bibliography

3. Phospho IRF-7 (Ser477/Ser479): general introduction
Interferon regulatory factor 7, known as IRF-7, belongs to the IRF family and is a key transcription factor that regulates anti-viral defenses by controlling the induction of type-I interferon (IFN) responses in Toll-like receptor pathway.
IRF-7 is preferentially expressed in lymphoid cells and induced by virus, lipopolysaccharide (LPS) and IFNα.
In response to infection, IRF-7 is activated by phosphorylation on Serine 477/Serine 479 through the action of Iκκε and TANK-binding kinase 1 (TBK-1).
Activation of IRF-7 leads to nuclear retention, DNA binding, and activation of IFNα promoters

4. Phospho IRF-7 (Ser477/Ser479): signaling pathway
Associated therapeutic areas :
Pathogen defenses
Autoimmunity
Inflammatory diseases

5. Phospho IRF-7 (Ser477/Ser479): assay format
Cell stimulation: triggers activation
Cell lysis: releases the phosphorylated kinase
Kinase detection: detects the phosphorylated IRF-7 in a sandwich assay format using an anti-phospho IRF-7 antibody labeled with Eu3+-Cryptate and an anti-IRF-7 antibody labeled with d2

6. Phospho IRF-7 (Ser477/Ser479): product specifications
Detection of endogenous level of IRF-7 phosphorylated on Ser 477/Ser479 in cell lysates
Robustness of the assay
High assay window
Cross-reactivity tested: Human

7. Phospho IRF-7 (Ser477/Ser479): assay performance
Positive lysates: treated to induce activation of IRF-7 in cells
Negative lysates: untreated cell lysates

8. Phospho IRF-7 (Ser477/Ser479): assay performance
Part number
Description name
Number of tests per kit
List price
63ADK020PEG
pIRF7 (Ser477/Ser479) tests
500
$1,450
63ADK020PEH
pIRF7 (Ser477/Ser479) - 10,000 tests
10,000
$7,900

9. Bibliography of interest:
IRF-7 is the master regulator of type-I interferon-dependent immune responses - Kenya Honda & al. | Nature 434, (7 April 2005)
Induction of IRF-3 and IRF-7 phosphorylation following activation of the RIG-I pathway - Paz S & al.| Cell Mol Biol (Noisy-le-grand) May 15;52(1):17-28.
Role of a transductional–tanscriptional processor complex involving MyD88 and IRF-7 in Toll-like receptor signaling - Honda K & al. | Proc Natl Acad Sci U S A. 2004; 101(43):
Interferon-α induction through Toll-like receptors involves a direct interaction of IRF7 with MyD88 and TRAF6 - Kawai T & al. | Nat Immunol. 2004;5(10):

10. Thank you