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Confocal microscope images of Reelin mRNA detected by in situ hybridization using a biotinylated oligoprobe (base pairs 1264–1311) visualized with fluorescein.

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Presentation on theme: "Confocal microscope images of Reelin mRNA detected by in situ hybridization using a biotinylated oligoprobe (base pairs 1264–1311) visualized with fluorescein."— Presentation transcript:

1 Confocal microscope images of Reelin mRNA detected by in situ hybridization using a biotinylated oligoprobe (base pairs 1264–1311) visualized with fluorescein (green) and GAD67 immunolabeling visualized with rhodamine (red) in adult rat neocortex ... Confocal microscope images of Reelin mRNA detected by in situ hybridization using a biotinylated oligoprobe (base pairs 1264–1311) visualized with fluorescein (green) and GAD67 immunolabeling visualized with rhodamine (red) in adult rat neocortex (Upper) and hippocampus (Lower). The overlay of the Reelin and GAD67 distribution shows that in the cortex mRNA for Reelin is found in both GABAergic and non-GABAergic cells, whereas in the hippocampus (CA1) all Reelin-positive cells are GABAergic. Similar results were obtained with Reelin antisense strand oligoprobe (base pairs 10225–10282). C. Pesold et al. PNAS 1998;95:6: ©1998 by National Academy of Sciences

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