1. Fall 2018
MCB 151

2. Course Information
MCB 151 Website: Login: NetID Password: AD password

4. Required Materials Required Texts and Supplies
1. "Laboratory Exercises for MCB 151: Molecular & Cellular Biology, Fall 2018" Melissa Reedy, editor
Stipes Publishing
 2. Biology Laboratory Notebook (carbonless)
Recommended (Optional) Texts
Textbook ISBN is: Biological Science (Freeman et. al, 6th edition) - currently used in MCB 150

5. Add, Drop, Section Change
Students may use the UI–Integrate Self-Service System to add or change MCB 151 laboratory sections before 5:00 PM, Monday, September 10, 2018
Friday, October 19 is the last day to drop the course or to elect the Credit/No Credit option. Students may use the UI-Integrate Self- Service System to drop MCB 151 until this deadline.

6. Course Grading (1000 points total)
Pre-Laboratory Exercises
9 7 points each, 50 points (drop 13 points)
Post-Laboratory Exercises*
10 15 points each, 125 points (drop 25 points)
Laboratory Reports
75 points each, 525 points (drop 75 points)
Laboratory Exams
100 points each
Final Exam
100 points
*Only students that are present and on time are eligible to complete the Post-Lab Exercise and Lab Report for that particular week. If you are absent/late, you may complete the Post-Lab Exercise for your own benefit (i.e. preparation for exams), but you will NOT earn points for it. If you are absent/late, you are not eligible to complete the Lab Report for that particular week and you may not be eligible to submit the previous weeks Lab Report.

7. Reading Assignments
The Reading Assignment for each week can be found on the course web site under the Laboratory Exercises link. These readings supplemental, all material will come from the lab manual and lab experience.

11. LON-CAPA
You will have Pre/Post Lab Assignments each week. Please make sure to follow the directions on pgs. I-18 your lab manual.

12. LON-CAPA

14.
In an emergency in this building, we’ll have three choices: RUN (get out), HIDE (find a safe place to stay inside), or FIGHT (with anything available to increase our odds for survival). First, take a few minutes this week and learn the different ways to leave this building. If there’s ever a fire alarm or something like that, you’ll know how to get out, and you’ll be able to help others get out too. Second, if there’s severe weather and leaving isn’t a good option, go to a low level in the middle of the building, away from windows. If there’s a security threat, such as an active shooter, we’ll RUN out of the building if we can do it safely or we will HIDE by finding a safe place where the threat cannot see us. We will lock or barricade the door and we will be as quiet as possible, which includes placing our cell phones on silent. We will not leave our area of safety until we receive an Illini-Alert that advises us it is safe to do so. If we cannot run out of the building safely or we cannot find a place to hide, we must be prepared to fight with anything we have available in order to survive. Remember, RUN away or HIDE if you can, FIGHT if you have no other option. Finally, if you sign up for emergency text messages at emergency.illinois.edu, you’ll receive information from the police and administration during these types of situations. If you have any questions, go to police.illinois.edu, or call

15. Emergencies in the lab Eye Wash Fire Extinguishers Fire Alarms
First Aid

16. Course Coordinator
Melissa Reedy Course Coordinator MCB 150/151 Office: 415 Burrill Hall Phone: Office hours: By appointment

17. Lab Techniques and Safety Introduction: Basic Skills
Exercise 1
Lab Techniques and Safety
Introduction:
Basic Skills

18. Laboratory Safety Bacteria: Chemicals: UV Light: Wear gloves
Wash you hands before leaving the lab
Notify your TA of any spills or contamination
Chemicals:
Always handle reagents with caution
UV Light:
Never look at UV lamps
Wear protective gloves

19. Laboratory Techniques
Clean up: each student is responsible for cleaning up after every experiment Labels: always label your experimental work with your name and section, b/c we share incubators, refrigerators, water baths, etc. Pipetting: always review the proper use of pipettes before beginning experiments Centrifuges: used to spin down and separate

20. Lab Safety Importance
Work you are doing now can have consequences now and in the future
Toxic or infectious substances could result in acute or chronic illness or even death.
Explosions or fires can injure or kill.
Knowledge of safety is critical in protecting yourself and others from potential hazards.

21. Housekeeping
Bench tops, floors and other surfaces must be kept clean and free of clutter
Coats and backpacks must be hung on coat hooks in designated areas
To avoid accidental ingestion of toxic or infectious materials, the following are prohibited in the lab:
Eating or drinking
Taking medication
Applying lip balm, gloss, lipstick or make-up

22. Hand Hygiene Wear gloves in the lab when directed to do so
Make sure to choose gloves that are the appropriate size and fit well
Do not use gloves that have holes or cracks
Always remove gloves before leaving the room
Remove gloves by pulling cuff down over your hand, turning the glove inside out
Never touch door knobs or handles with gloved hands
Always wash hands after removing gloves

23. Personal Protective Equipment
Students may elect to wear safety glasses or goggles, which are recommended but not required
Students may also elect to wear lab coats
PPE can be purchased from:
Life Sciences storeroom, C107 CLSL, phone:
RAL storeroom, 94 RAL, phone:

24. Personal Attire in the Lab
The following guidelines apply to attire in the lab:
Clothing should cover legs and midriff
Footwear should completely cover feet (no open-toed shoes or sandals)
Long hair should be tied back
Loose sleeves or tops should be confined

25. Biosafety Levels
Biosafety is the application of a combination of laboratory practices and procedures, laboratory facilities, and safety equipment when working with biological material
Biosafety levels:
BL1 - Agents not known to cause disease consistently in healthy humans
BL2 - Agents associated with human or animal disease
BL3 - Indigenous/exotic agents associated with human or animal disease and with potential for aerosol transmission
BL4 - Dangerous/exotic agents of life threatening nature

26. Sharps The Illinois EPA defines sharps as: Any medical needle
Syringe, with or without needle
Scalpel and razor blade
Blood vials
Pasteur pipets
Microscope slides and coverslips
Glassware contaminated with infectious agents
Sharps must be disposed of in approved Sharps container

27. Biological Waste
Cultures, stocks and disposable labware generated from experiments with biological materials must be autoclaved prior to disposal
Place these materials, as directed, into red biological waste disposal bags in the lab
Do NOT put these materials into regular trash
If you are uncertain, please ask!

28. Emergency Preparedness
Please note the location of the following:
Shower
Eyewash
Fire extinguisher
Spill kit
Nearest exit
If you must, evacuate to prevent or minimize injury

29. Fire Safety If fire occurs: Notify those in the immediate area
Activate nearest fire alarm
Confine the fire by closing doors and windows
Attempt to extinguish the fire only if it is safe to do so
Do not use elevators
If possible, call 911 to explain the nature of the fire or emergency and meet responders outside

30. DRS
The DRS website provides campus-specific safety information such as:
Safety guidance documents in the "Safety Library”
Online Safety Trainings
Information on how to manage hazardous waste
Information on DRS safety programs
Accident response information
Templates and forms for managing hazards in the laboratory

31. Exercise 1B: Basic Laboratory Skills,
Exercise 1B: Basic Laboratory Skills

32. Goals Understand and manipulate exponential numbers.
Become familiar with the units used for small concentrations and volumes and be able to make conversions between them.
Figure out concentrations of solutions or cell suspensions after dilution.
Understand what is meant by pH and know how to calculate the pH of a solution.
Understand how buffers work.

33. Exponential Numbers
Every number can be expressed as the product of two numbers, the second one being some power of ten.
For example, the number 100 can be expressed as X 10 = 102
The same rules are used to express numbers less than one.
10-2 = 1/10 X 1/10 = 1/100

34. Units of Measurement
Common units Mass 1 gram (g) = 103 milligrams (mg) = 106 micrograms (µg) = 109 nanograms (ng) Volume 1 liter (l) = 103 milliliters (ml) = 106 microliters (µl) 1 ml = 1 cubic centimeter Length 1 meter (m) = 103 millimeters (mm) = 106 micrometers (µm) = 109 nanometers (nm)

35. Dilutions
C1V1 = C2V2
Example: Given a 1 M stock solution of Tris buffer prepare ml of 0.01 M Tris.
Solution: The question you need to answer is "what volume of 1 M Tris do you need to dilute to give a total volume of 10 ml of 0.01 M Tris". Thus, you know C1, C2, and V2.
(? ml x 1 M) = (10 ml x 0.01 M)
? ml = 10 ml x 0.01 M
(1 M)
Answer: 0.1 ml

36. Dilution Factor
Dilution = 1/100 Dilution Factor: 100 Dilution = 1/n Dilution Factor: n Serial Dilution: product of the individual dilutions 1/102 x 1/10 x 1/103 = 1/106 = 10-6

37. An aqueous solution is one in which water is the solvent.
Aqueous Solutions
An aqueous solution is one in which water is the solvent.
Water properties:
High surface tension
High boiling point
Good Solvent

38. Acids, Bases, pH and Buffer
Acids/Bases: Bronsted Definition: an acid is a molecule that donates protons and a base is a molecule that accepts protons pH: Dependent upon the concentrations of H+ and OH- ions Buffers: A molecule that allows the solutions to resist changes in the hydrogen ion concentration

39. Assignments for next week
Post Lab 1 and Pre Lab 2 are assigned today and due before your lab meets again in LON-CAPA.
You must come to lab next week with your pre lab write up complete for Exercise 2B Parts 1 and 2
Purpose
Procedure/Protocol