1. Review of Castleton Microarray Images and QC
Why do we look at this image?
-Good for seeing visual defects
-Examining Borders, Chip ID, Controls
Why do we look at the QC report?
Check 3’ to 5’ ratios of housekeeping genes
-Scaling factor
-Spike in control signal
So lets look at the images and see what happened…
team by team

2. CSC1-CONTROL A
GOOD !!

3. CSC2-FERM
GOOD !!

4. CSC3-CONTROL B
GOOD !!

5. CSC4-DMSO A
GOOD !!

6. CSC5-CONTROL A
GOOD !!

7. CSC6-DMSO A
No Good-Failed Rxn 
Just kidding
GOOD !! 

8. QC Report From Genechip
Actin Housekeeping Control 3’-5’ Ratio
TATA BP