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DNA Extraction Lab
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Here is the level of organization of all living things
Let’s start with the cell
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Cells come together to make tissues
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Tissues come together to form organs
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Organs work together to make organ systems
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Organ systems work together to form an organism
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Let’s review… cell tissue organ Organ system organism
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Back to cells Animal Cell Plant Cell Where is the Dna? In the nucleus
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First, we Go into the cell
Get the dna Break through the membranes
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Here is what we will need…
Meat tenderizer water Dishwashing soap bananas salt Ethyl alcohol
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Breaks through the membranes
What does each do? Dishwashing soap Breaks through the membranes
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Breaks down proteins in meat to make tender
Meat tenderizer Yum!! Little proteins in dna The meat tenderizer dissolves proteins that hold dna together and lets it unwind.
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Separates the dna from the unwanted cell parts
salt Unwanted cell parts dna Separates the dna from the unwanted cell parts
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Dna does NOT dissolve in alcohol
Dna dissolves in water water Dna does NOT dissolve in alcohol Ethyl alcohol
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What lab equipment will we need?
microcentrifuge Test tubes Graduated cylinder paperclips Test tube holder
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Let’s begin!
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