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Project Idea: Biofuels

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Presentation on theme: "Project Idea: Biofuels"— Presentation transcript:

1 Project Idea: Biofuels
Stanford iGEM 2009 Presentation by: Suzie + Robert 3/4/09

2 Biofuels Introduction
What makes a good biofuel?!?! Environmentally friendly Mass production - CHEAP! Chemically: High energy density Low water solubility

3 Biofuels Introduction

4 Biofuels Introduction
As for synthetic biology applications… Most use E. coli or S. cerevisiae as a host to: 1. Engineer amino acid biosynthesis 2. Engineer fatty acid pathways 3. Engineer isoprenoid pathways

5 Dr. James Liao

6 Dr. James Liao

7 3-Methyl-1-Butanol

8 2-Methyl-1-Butanol

9 Dr. James Liao

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13 Our Project We would need to engineer a separate amino acid pathway to produce a possible biofuel EX: Serine or alanine Need to ID enzymes that can make this possible: For Dr. Liao, it was KIVD (yeast) and ADH6 (lactococcus lactis)

14 Five Possible Sub-projects
1. Synthesizers (constructing the pathway) 2. Riboregulators (controlling knockouts) 3. Modelers (Modeling for bottlenecks) 4. Bottleneckers (Building expression system) 5. ANY OF THE ABOVE

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