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Project Idea: Biofuels
Stanford iGEM 2009 Presentation by: Suzie + Robert 3/4/09
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Biofuels Introduction
What makes a good biofuel?!?! Environmentally friendly Mass production - CHEAP! Chemically: High energy density Low water solubility
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Biofuels Introduction
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Biofuels Introduction
As for synthetic biology applications… Most use E. coli or S. cerevisiae as a host to: 1. Engineer amino acid biosynthesis 2. Engineer fatty acid pathways 3. Engineer isoprenoid pathways
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Dr. James Liao
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Dr. James Liao
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3-Methyl-1-Butanol
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2-Methyl-1-Butanol
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Dr. James Liao
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Our Project We would need to engineer a separate amino acid pathway to produce a possible biofuel EX: Serine or alanine Need to ID enzymes that can make this possible: For Dr. Liao, it was KIVD (yeast) and ADH6 (lactococcus lactis)
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Five Possible Sub-projects
1. Synthesizers (constructing the pathway) 2. Riboregulators (controlling knockouts) 3. Modelers (Modeling for bottlenecks) 4. Bottleneckers (Building expression system) 5. ANY OF THE ABOVE
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