1. Department of Genetic Ecotoxicology topic: molecular epidemiology
Radim J. SRAM
Institute of Experimental Medicine Academy of Sciences of the Czech Republic
Department of Genetic Ecotoxicology topic: molecular epidemiology

2. Monthly concentrations of benzo[a]pyrene10 15 20 25 30 35 40 5
Jan
Feb
Mar
Apr
May
Jun
Jul
Aug
Sept
Oct
Nov
Dec
Monthly concentrations of benzo[a]pyrene
(Ostrava - Bartovice)
ng/m3
Impact on DNA
fragmentation in sperm
Impact of pregnancy
outcome
limit/year
( ): 1 ng/m3
Ostrava - Region in the Czech Republic is highly polluted by c-PAHs from industry (B[a]P up to 7-10 ng/m3/year)
asthma bronchiale
in children
It is related to the
significant increase of
genetic and oxidative damage in adults
We may expect the:
impact on pregnancy outcome
as well as
the children mental development
This high exposure should decrease within 3-5 next years. This difficult situation should be used to an international study to analyze the health risk for population as well as the effect of remediation.
R. J. Sram 2011

3. CHILDREN RESPIRATORY MORBIDITY
(URI +
bronchitis + laryngitis + pneumonia +
tonsillitis
otitis
media) 7 6 5 4
M.O. (413)
Hrab. (595)
Zabr. (348)
Por. (313)
R. a B. (154)
Incidence/child 3 2 1
1st
2nd
3rd
4th
5th
6th
Years of age
Our skills:
biomarkers of exposure (DNA adducts), of effect (micronuclei, oxidative damage), of susceptibility (gene expression, DNA methylation, genetic polymorphisms )
Idea:
this specific situation could be used to analyze the long term impact of air pollution, e.g.:
gene expression and DNA methylation in mothers and newborns, impact to their development
specificity of observed respiratory morbidity in children to air pollution
effect of c-PAHs exposure to the mental development of children
impact of oxidative damage in adults to cardiovascular morbidity
analysis of dose-effect relationship for the health risk assessment
R. J. Sram 2011