1. The effect of molecular weight on hyaluronan’s cartilage boundary lubricating ability – alone and in combination with proteoglycan 4 
J.J. Kwiecinski, S.G. Dorosz, T.E. Ludwig, S. Abubacker, M.K. Cowman, T.A. Schmidt 
Osteoarthritis and Cartilage 
Volume 19, Issue 11, Pages (November 2011)
DOI: /j.joca
Copyright © 2011 Osteoarthritis Research Society International Terms and Conditions

2. Fig. 1
MW characterization & confirmation of HA used in test lubricants (20kDa, 132kDa, 780kDa, 1.5MDa, 5MDa) via AGE and staining, as described in the methods.
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2011 Osteoarthritis Research Society International Terms and Conditions

3. Fig. 2
Effect of MW of HA alone on the boundary lubrication of articular cartilage. Test 1 (n=5): 132kDa, 780kDa, 1.5MDa (A, B); Test 2 (n=5): 20kDa, 780kDa, 5MDa (C, D). Shown are static μstatic, Neq (A, C), and kinetic <μkinetic, Neq> (the brackets indicate that the value is an average) (B, D) friction coefficients in PBS, PBS+various MW HA at 3.33mg/ml, and SF. In B, D, the pre-sliding duration, Tps, was 1.2s. Data are mean±95% CI.
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2011 Osteoarthritis Research Society International Terms and Conditions

4. Fig. 3
Dependence of the cartilage boundary lubricating properties of HA alone at 3.33mg/ml on MW. Regression lines are shown for mean static μstatic, Neq friction values at pre-sliding duration, Tps=1,200 s (A), and mean kinetic <μkinetic, Neq> friction values at Tps=1.2s (B) obtained from HA Test 1 & 2 (Fig. 2) vs log MW HA. Mean values in PBS and SF are shown for reference.
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2011 Osteoarthritis Research Society International Terms and Conditions

5. Fig. 4
Effect of MW of HA when combined with PRG4 on the boundary lubrication of articular cartilage. Test 1 (n=5): PRG4, PRG4+132kDa, PRG4+1.5MDa (A, B) Test 2 (n=5): PRG4, PRG4+20kDa, PRG4+5MDa (C, D). Shown are static μstatic, Neq (A, C), and kinetic <μkinetic, Neq> (B, D) friction coefficients in PBS, PBS+PRG4 at 450μg/ml, PBS+PRG4± various MW HA at 3.33mg/ml, and SF. In B, D the pre-sliding duration, Tps, was 1.2s. Data are mean±95% CI.
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2011 Osteoarthritis Research Society International Terms and Conditions

6. Fig. 5
Dependence of the cartilage boundary lubricating properties of PRG4+HA, at 450μg/ml and 3.33mg/ml respectively, on HA MW. Regression lines are shown for mean static μstatic, Neq friction values at pre-sliding duration, Tps=1,200s (A), and mean kinetic <μkinetic, Neq> friction values at Tps=1.2s (B) obtained from HA+PRG4 Test 1 & 2 (Fig. 4) vs log MW HA. Mean values in PBS, PRG4 at 450μg/ml, and SF are shown for reference.
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2011 Osteoarthritis Research Society International Terms and Conditions

7. Fig. 6
EMSA of 1μg 150kDa (A) and 1,000kDa (C) monodisperse HA with graded amounts of PRG4 (0, 1, 10μg shown), and densitometric analysis of migration distance of 150kDa (B) and 1,000kDa (D) HA, as described in the methods. PRG4:HA molar ratios are calculated using an approximate average MW of PRG4=400kDa.
Osteoarthritis and Cartilage  , DOI: ( /j.joca )
Copyright © 2011 Osteoarthritis Research Society International Terms and Conditions

8. Osteoarthritis and Cartilage 2011 19, 1356-1362DOI: (10. 1016/j. joca
Copyright © 2011 Osteoarthritis Research Society International Terms and Conditions

9. Osteoarthritis and Cartilage 2011 19, 1356-1362DOI: (10. 1016/j. joca
Copyright © 2011 Osteoarthritis Research Society International Terms and Conditions