1. Restriction Enzymes Lecture
2014

2. An enzyme that cuts DNA at a specific reaction site (sequence)
What is a restriction enzyme?
An enzyme that cuts DNA at a specific reaction site (sequence)

3. Examples of restriction enzymes
Blunt Ends
“Sticky” Ends

4. Restriction enzymes destroy invading bacteriophages
Restriction enzymes are found in bacteria
Restriction enzymes destroy invading bacteriophages
BamHI destroys Bacillus amyloliquefaciens
HindIII destroys Haemophilus influenza

5. Scientists build designer plasmids with specific restriction sites
Restriction enzymes in biotechnology
Scientists build designer plasmids with specific restriction sites

6. Recombined genes are cloned and expressed
Restriction enzymes in biotechnology
Recombined genes are cloned and expressed

7. Many restriction sites are often palindromes
Palindromes are the same forwards/backward:
A nut for a jar of tuna
Eva, can I stab bats in a cave

8. Palindromes result in sticky ends, which form hydrogen bonds easily

9. Laboratory 3: Ligation sticky end sticky end sticky end sticky end
BamHI 3′
5 ′
3 ′
sticky end
HindIII
5 ′
sticky end
HindIII 5′ 3′ 5′ 5′ 3′
sticky end
BamHI 3′ 3′ 5′