Presentation is loading. Please wait.

Presentation is loading. Please wait.

Constrictive external nitinol meshes inhibit vein graft intimal hyperplasia in nonhuman primates  Peter Zilla, MD, PhD, Paul Human, PhD, Michael Wolf,

Published byRalf Welch Modified over 6 years ago

Similar presentations

Presentation on theme: "Constrictive external nitinol meshes inhibit vein graft intimal hyperplasia in nonhuman primates  Peter Zilla, MD, PhD, Paul Human, PhD, Michael Wolf,"— Presentation transcript:

1 Constrictive external nitinol meshes inhibit vein graft intimal hyperplasia in nonhuman primates 
Peter Zilla, MD, PhD, Paul Human, PhD, Michael Wolf, BSc, Wilhelm Lichtenberg, MB ChB, Nasser Rafiee, BSc, Deon Bezuidenhout, PhD, Nazlia Samodien, BTechHons, Christian Schmidt, MD, Thomas Franz, PhD  The Journal of Thoracic and Cardiovascular Surgery  Volume 136, Issue 3, Pages (September 2008) DOI: /j.jtcvs Copyright © 2008 The American Association for Thoracic Surgery Terms and Conditions

2 Figure 1 Image analysis of cross sections of pressure-fixed, explanted grafts by (a) manual delineation and quantification of anatomic zones on tiled stitched elastin-Masson trichrome images followed by (b) quantification of vasa vasorum within these zones on tiled factor VIII immunofluorescence images. The entire high-resolution image, a section of which is shown in (c), was translated into vessel area data for each zone after hue saturation and intensity detection (d). IH, Intimal hyperplasia tissue. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs ) Copyright © 2008 The American Association for Thoracic Surgery Terms and Conditions

3 Figure 2 Change in cross-sectional luminal area. Preimplant values reflect the luminal area of in situ BSVs and were excluded from inferential statistical analysis of the remaining time points. Non–mesh-supported control grafts underwent a significant narrowing of the lumen during the initial 6 weeks followed by a marked dilatation thereafter. The mild trend toward luminal enlargement in the mesh-supported grafts is a reflection of mesh dilatation. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs ) Copyright © 2008 The American Association for Thoracic Surgery Terms and Conditions

4 Figure 3 Non-dewired midgraft sections representing all 8 groups. (Resin; precision sawed and ground; hematoxylin and eosin stain, 0.5×lense) The circles inserted in the control group highlight the degree of shrinkage during the initial 6 weeks and the extent of dilatation thereafter. In the 6.7-mm mesh-supported group and to a mild degree in the 5.0-mm group, the gap between the shrunken vein grafts and the mesh is filled with fibrous tissue. Note the delicate thin wall of veins inside the 50% diameter-constrictive 3.3-mm meshes. There is a shallow longitudinal fold in the 12-week section. Numbers inserted in the left upper corner represent the percentage diameter dilatation of the meshes when compared with their dimensions at implantation. N.S., Not significant. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs ) Copyright © 2008 The American Association for Thoracic Surgery Terms and Conditions

5 Figure 4 Macroscopic comparison of 6-week vein grafts supported by 6.7-mm meshes (left; a and c) and 3.3-mm meshes (right; b and d). In the 6.7-mm mesh-supported group, there is clear distinction between the more whitish vessel wall and the fibrous tissue filling the gap between vessel wall and mesh. Note the delicate vessel wall inside the 3.3-mm mesh. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs ) Copyright © 2008 The American Association for Thoracic Surgery Terms and Conditions

6 Figure 5 Serial sections of dewired midgraft regions (controls a–c/size-matched 6.7-mm mesh support: d–f and 50% diameter constrictive 3.3-mm mesh support g–i) stained with modified elastic Masson's trichrome (left and middle column) and modified Movat's (right column). Small rectangles indicate high magnification areas shown in middle column (20× objective); larger rectangles represent areas shown in right column (10× objective). Pronounced, excentric glucosaminoglycans-rich (blue) intimal hyperplasia with discernible internal elastic lamella (↑) and loose collagen-rich media in controls and the size-matched group. Distinct (50%) downsizing abolished intimal hyperplasia tissue (IH) and led to a thin, compacted media. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs ) Copyright © 2008 The American Association for Thoracic Surgery Terms and Conditions

7 Figure 6 Cross-sectional area of intimal hyperplasia tissue. The bulk of proliferation occurs during the initial 6 weeks. The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs ) Copyright © 2008 The American Association for Thoracic Surgery Terms and Conditions

Download ppt "Constrictive external nitinol meshes inhibit vein graft intimal hyperplasia in nonhuman primates  Peter Zilla, MD, PhD, Paul Human, PhD, Michael Wolf,"

Similar presentations

In vitro tissue engineering of a cardiac graft using a degradable scaffold with an extracellular matrix–like topography  Osamu Ishii, MD, Michael Shin,

In vitro tissue engineering of a cardiac graft using a degradable scaffold with an extracellular matrix–like topography  Osamu Ishii, MD, Michael Shin,
Lost in translation The Journal of Thoracic and Cardiovascular Surgery

Lost in translation The Journal of Thoracic and Cardiovascular Surgery
Bioengineered vascular access maintains structural integrity in response to arteriovenous flow and repeated needle puncture  Bryan W. Tillman, MD, PhD,

Bioengineered vascular access maintains structural integrity in response to arteriovenous flow and repeated needle puncture  Bryan W. Tillman, MD, PhD,
Reconstruction of pulmonary artery with porcine small intestinal submucosa in a lamb surgical model: Viability and growth potential  Lorenzo Boni, MD,

Reconstruction of pulmonary artery with porcine small intestinal submucosa in a lamb surgical model: Viability and growth potential  Lorenzo Boni, MD,
Anti–VLA-4 antibody reduces intimal hyperplasia in the endarterectomized carotid artery in nonhuman primates  Alan B. Lumsden, MB, ChB, Changyi Chen,

Anti–VLA-4 antibody reduces intimal hyperplasia in the endarterectomized carotid artery in nonhuman primates  Alan B. Lumsden, MB, ChB, Changyi Chen,
Three arteries versus the saphenous vein for coronary artery bypass graft: Why use a damaged graft to repair a damaged heart?  Andrzej Loesch, PhD, DSc,

Three arteries versus the saphenous vein for coronary artery bypass graft: Why use a damaged graft to repair a damaged heart?  Andrzej Loesch, PhD, DSc,
Peter Zilla, MD, PhD, Loven Moodley, FCS(SA), Michael F

Peter Zilla, MD, PhD, Loven Moodley, FCS(SA), Michael F
Transcatheter valve insertion in a model of enlarged right ventricular outflow tracts  Adeline Basquin, MD, Emmanuelle Pineau, MD, Louise Galmiche, MD,

Transcatheter valve insertion in a model of enlarged right ventricular outflow tracts  Adeline Basquin, MD, Emmanuelle Pineau, MD, Louise Galmiche, MD,
Tracheal regeneration: Evidence of bone marrow mesenchymal stem cell involvement  Agathe Seguin, MD, PhD, Sonia Baccari, MD, Muriel Holder-Espinasse, MD,

Tracheal regeneration: Evidence of bone marrow mesenchymal stem cell involvement  Agathe Seguin, MD, PhD, Sonia Baccari, MD, Muriel Holder-Espinasse, MD,
Decellularization reduces immunogenicity of sheep pulmonary artery vascular patches  Eric J. Lehr, MD, PhD, Gina R. Rayat, PhD, Brian Chiu, MD, Thomas.

Decellularization reduces immunogenicity of sheep pulmonary artery vascular patches  Eric J. Lehr, MD, PhD, Gina R. Rayat, PhD, Brian Chiu, MD, Thomas.
Remodeling leads to distinctly more intimal hyperplasia in coronary than in infrainguinal vein grafts  Peter Zilla, MD, PhD, Loven Moodley, MBBS, FCS,

Remodeling leads to distinctly more intimal hyperplasia in coronary than in infrainguinal vein grafts  Peter Zilla, MD, PhD, Loven Moodley, MBBS, FCS,
Decellularized heart valve as a scaffold for in vivo recellularization: Deleterious effects of granulocyte colony-stimulating factor  Francis Juthier,

Decellularized heart valve as a scaffold for in vivo recellularization: Deleterious effects of granulocyte colony-stimulating factor  Francis Juthier,
On-pump versus off-pump: It's all about the grafts!

On-pump versus off-pump: It's all about the grafts!
Targeted imaging of matrix metalloproteinase activity in the evaluation of remodeling tissue-engineered vascular grafts implanted in a growing lamb model 

Targeted imaging of matrix metalloproteinase activity in the evaluation of remodeling tissue-engineered vascular grafts implanted in a growing lamb model 
Thomas J. van Brakel, MD, PhD, Paul H

Thomas J. van Brakel, MD, PhD, Paul H
Right internal thoracic artery or saphenous vein grafting

Right internal thoracic artery or saphenous vein grafting
A composite semiresorbable armoured scaffold stabilizes pulmonary autograft after the Ross operation: Mr Ross's dream fulfilled  Francesco Nappi, MD,

A composite semiresorbable armoured scaffold stabilizes pulmonary autograft after the Ross operation: Mr Ross's dream fulfilled  Francesco Nappi, MD,
Utilization of shape memory in external vein-graft meshes allows extreme diameter constriction for suppressing intimal hyperplasia: A non-human primate.

Utilization of shape memory in external vein-graft meshes allows extreme diameter constriction for suppressing intimal hyperplasia: A non-human primate.
Long-term experience in autologous in vitro endothelialization of infrainguinal ePTFE grafts  Manfred Deutsch, MD, Johann Meinhart, PhD, Peter Zilla,

Long-term experience in autologous in vitro endothelialization of infrainguinal ePTFE grafts  Manfred Deutsch, MD, Johann Meinhart, PhD, Peter Zilla,
Targeted overexpression of leukemia inhibitory factor to preserve myocardium in a rat model of postinfarction heart failure  Mark F. Berry, MD, Timothy.

Targeted overexpression of leukemia inhibitory factor to preserve myocardium in a rat model of postinfarction heart failure  Mark F. Berry, MD, Timothy.

Similar presentations

Ads by Google