1. SDS-PAGE and immunoblot analysis of F4:5 progeny of a 10/81b × PI 603408 cross.
SDS-PAGE and immunoblot analysis of F4:5 progeny of a 10/81b × PI cross. (A) SDS-PAGE analysis of total seed proteins and (B) immunoblot analysis of total seed proteins with anti-glycinin 5 antibodies. Samples 1, 13, and 14 are from line Patriot and sample 2 is seed from PI plants. Samples 3–12 are selected progeny from a 10/81b × PI cross. The genotype of plant that produced seed is indicated below figure: “+” indicates homozygosity for wild-type alleles, “H” indicates heterozygosity, and “−” indicates homozygosity for mutant alleles. SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis.
Jason D. Gillman et al. G3 2017;7:
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