1. Phosphatase activity of PGAM5 is required for activation of ASK1.
Phosphatase activity of PGAM5 is required for activation of ASK1. (A) Phosphatase activity of PGAM5 is required for ASK1 activation. HA-ASK1-Δcoil was coexpressed with Flag-PGAM5-WT, -H/A, or -H/F in HEK293 cells. Cell lysates were subjected to immunoblotting (IB). P-ASK antibody recognizes phosphorylation of human ASK1 Thr-838. (B) Phosphatase activity of PGAM5 is required for activation of JNK and p38. PGAM5-WT, -H/A, and -H/F were individually expressed in HEK293 cells. Cell lysates were subjected to IB.
Kohsuke Takeda et al. PNAS 2009;106:30:
©2009 by National Academy of Sciences