1. A Control B AD C Control D AD Non immunodepletion Non immunodepletion
LPS immuno-depleted
LPS immuno-depleted
Figure e-2. Immunoabsorption specificity controls in AD and control brain. Immunofluorescence of AD and control brains using antibody against E.coli LPS (Abcam, ab35654) as well as E. coli LPS depleted antibody (LPS from VWR, ; antibody:LPS = 1:10). The anti-E.coli LPS antibody detected nuclei in control (A) and AD (B) brains as well as plaques in AD brains (B), whereas E. coli LPS immuno-depleted antibody almost completely eliminated all tissue staining in control brain (C) and AD brain (D). These data showed that LPS was being immunostained. AD = Alzheimer’s disease. Bar = 50µm.