1. Antisense oligonucleotides against the Bcl-2 transcript reversed the cytoprotective effects of DHEA(S) and Allo on serum-deprived PC12 cells.
Antisense oligonucleotides against the Bcl-2 transcript reversed the cytoprotective effects of DHEA(S) and Allo on serum-deprived PC12 cells. Cells were cultured for 24 h in serum-free media containing 10–7 M DHEA, DHEAS, or Allo in the absence or the presence of 50 μmol per liter of nonsense (NS) or antisense (AS) 20-mer phosphorothioate oligodeoxynucleotides directed toward the translation-initiation site of the Bcl-2 transcript. Apoptosis was quantified by the APOPercentage assay (A) and the Bcl-2 proteins by Western blot (B). Values represent the mean ± SD of three independent experiments (*, P < 0.05); S, steroid; 1, Bcl-2; 2, actin).
Ioannis Charalampopoulos et al. PNAS 2004;101:21:
©2004 by National Academy of Sciences