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Christiane Kruse Fæste, PhD, Ellen Namork, PhD, Helene Lindvik, MMSc 

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Presentation on theme: "Christiane Kruse Fæste, PhD, Ellen Namork, PhD, Helene Lindvik, MMSc "— Presentation transcript:

1 Allergenicity and antigenicity of fenugreek (Trigonella foenum-graecum) proteins in foods 
Christiane Kruse Fæste, PhD, Ellen Namork, PhD, Helene Lindvik, MMSc  Journal of Allergy and Clinical Immunology  Volume 123, Issue 1, Pages (January 2009) DOI: /j.jaci Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 Coomassie-stained SDS-PAGE of legume extracts. M1, SeeBluePlus2 prestained molecular weight marker; F, fenugreek; 1, fenugreek; 2, peanut; 3, lupin; 4, pea; 5, soy; 6, bean; 7, lentil; 8, chickpea; 9, curry; 10, tikka masala; M2, Mark12 molecular weight marker. Protein sizes (kd) are indicated on the sides of the gel. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 Immunoblot using sera from fenugreek-sensitized patients (Table I). A, Fenugreek. B, Peanut. M1, Marker (kd). Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig 3 Immunoblot using sera from patients with fenugreek allergy (Table II). A, Patient A. B, Patient B. 1, Fenugreek; 2, peanut; 3, lupin; 4, pea; 5, soy; 6, bean; 7, lentil; 8, chickpea; 9, curry; 10, tandoori masala; 11, cinnamon; 12, Indian tikka masala; 13, naan authentic; M1, Marker (kd). Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig 4 Fenugreek in food products. Immunoblot using polyclonal rabbit antifenugreek antibody. 1-32, Food products in the same order as in Table III. M, M1 Marker (kd). Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6 Fig 5 A and B, Allergenicity of fenugreek-containing foods. Immunoblot using sera from patients differently sensitized to peanut and fenugreek. 1-32, Food products in the same order as in Table III. M, M1 marker (kd). Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

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