1. NG2-CreERT2 postnatal day 31
Hassouna et al Supplementary Figure 2: EPOR expression in OPC A C
200 µm
CA1 CC
GFP
EPOR
DAPI
NG2-CreERT2 postnatal day 31
qPCR primary mouse OPC
20 µm
GFP
DAPI
20 µm
GFP
EPOR
DAPI
20 µm
EPOR
DAPI B
EPOR Antibody (polyclonal rabbit, 400ng/ml) 1. 2. 3. 4.
EPOR Western Blot
70-
55-
35-
100-
kDa
70-
55-
35-
100-
kDa 1. 2. 3. 4.
HA Western Blot
Protein lysates (15 µg protein/lane):
1: HEK FT cells transfected with murine EPOR 2: EOC-20 cells transduced with HA-tagged
human EPOR
3: UT-7 cells (EPO dependent human cell line)
4: Control EOC-20 cells (non-transduced)
Bands:
Native full length EPOR
EPOR fragments