1. Acetylcholine/muscarinic signaling maintains SOX2 and the acinar lineage in human SG
Acetylcholine/muscarinic signaling maintains SOX2 and the acinar lineage in human SG AHuman salivary gland obtained from healthy individuals (no IR; submandibular) or patients who received radiation therapy for head and neck cancer (IR) were subjected to qPCR.B–DHuman SMG explants cultured for 7 days with either murine embryonic day (E) 13 parasympathetic ganglia (nerves) or E13 mesenchyme (MES). Explants were analyzed by immunostaining for markers of nerves (B, TUBB3) or cell proliferation (C, Ki67) or by qPCR (D). Scale bars = 50 μm (B, C).EqPCR analysis of adult human salivary gland (SMG or PG) explants of four different individuals cultured for 4 h ± CCh (200 nM, n = 4). Individual datasets are shown in Fig EV5D.Data information: Data in (A) n = 11 for no IR and n = 7 for IR; 30–85 years. Data were normalized to GAPDH with individual values plotted and analyzed using a Student's t‐test with a false discovery rate set to Error bars show mean ± SD. AQP3 P = 0.017, MIST1 P = 0.086, AMY1 P = 0.005, SOX2 P = 0.079, CHRM1 P = 0.040, CHRM3 P = 0.011, EGFR P = 0.931, KRT19 P =  Data in (D) are means + SD of n = 2 individuals where solid line columns represent individual #1 and broken line columns represent individual #2. Data were normalized to salivary glands from the same individuals cultured with murine mesenchyme (Control, black dashed line). Data in (E) are box and whisker plots of n = 4 different individuals, showing means (horizontal line), upper and lower quartiles (box) and upper and lower values (whiskers). Data were normalized to the untreated control (black dashed line). n = 5–8 explants per individual. Data were analyzed using a one‐way analysis of variance with post hoc Dunnett's test. SOX2 **P =  , CHRM3 *P = 0.0449, CHRM1 **P = 0.0093, AQP5 **P = 0.0069, AQP3 *P = 0.0375, MIST1 *P = 0.0379, CD44 *P = 0.0485, KRT19 *P = 
Elaine Emmerson et al. EMBO Mol Med. 2018;emmm
© as stated in the article, figure or figure legend