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In vitro lymphocyte proliferation with milk and a casein–whey protein hydrolyzed formula in children with cow's milk allergy  Philippe A. Eigenmann, MD,

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Presentation on theme: "In vitro lymphocyte proliferation with milk and a casein–whey protein hydrolyzed formula in children with cow's milk allergy  Philippe A. Eigenmann, MD,"— Presentation transcript:

1 In vitro lymphocyte proliferation with milk and a casein–whey protein hydrolyzed formula in children with cow's milk allergy  Philippe A. Eigenmann, MD, Dominique C. Belli, MD, Françoise Lüdi, Jean-Maurice Kahn, MD, Barbara S. Polla, MD  Journal of Allergy and Clinical Immunology  Volume 96, Issue 4, Pages (October 1995) DOI: /S (95) Copyright © 1995 Mosby, Inc. Terms and Conditions

2 FIG. 1 LPT with milk; results are expressed in cpm. Circles represent test 1 (baseline), and diamonds represent test 2 (at 3-month follow-up) for patients with CMA. Open symbols indicate patients with IgE-mediated symptoms, and solid symbols, patients with non-IgE-mediated symptoms. Squares represent the control group. The median for each group is shown by a bar. Counts per minutes are plotted on the y axis on a log scale. Controls and different concentrations of antigen are plotted on the x axis. Statistical analysis: †p values when results of test 1 are compared in patients with CMA and the control group; ‡p values when results of tests 1 and 2 are compared in patients with CMA. There is no statistical difference between patients with IgE-mediated symptoms and those with non-IgE-mediated symptoms. PHA, Phytohemagglutinin; TT, tetanus toxoid, n.s., not significant. Journal of Allergy and Clinical Immunology  , DOI: ( /S (95) ) Copyright © 1995 Mosby, Inc. Terms and Conditions

3 FIG. 2 LPT with casein; results are expressed in cpm. Circles represent test 1 (baseline), and diamonds represent test 2 (at 3-month follow-up) for patients with CMA. Open symbols indicate patients with IgE-mediated symptoms, and solid symbols, patients with non-IgE-mediated symptoms. Squares represent the control group. The median for each group is shown by a bar. Counts per minute are plotted on the y axis on a log scale. Controls and different antigen concentrations are plotted on the x axis. Statistical analysis: †p values when results of test 1 are compared in patients with CMA and the control group; ‡p values when results of tests 1 and 2 are compared in patients with CMA. There is no statistical difference between patients with IgE-mediated symptoms and those with non-IgE-mediated symptoms. PHA, Phytohemagglutinin; TT, tetanus toxoid, n.s., not significant. Journal of Allergy and Clinical Immunology  , DOI: ( /S (95) ) Copyright © 1995 Mosby, Inc. Terms and Conditions

4 FIG. 3 LPT with whey protein; results are expressed in cpm. Circles represent test 1 (at diagnosis), and diamonds represent test 2 (at 3-month follow-up) for patients with CMA. Open symbols indicate patients with IgE-mediated symptoms, and solid symbols, patients with non-IgE-mediated symptoms. Squares represent the control group. The median for each group is shown by a bar. Counts per minute are plotted on the y axis on a log scale. Controls and different antigen concentrations are plotted on the x axis. Statistical analysis: †p values when results of test 1 are compared in patients with CMA and the control group; ‡p values when results of tests 1 and 2 are compared in patients with CMA. There is no statistical difference between patients with IgE-mediated symptoms and those with non-IgE-mediated symptoms. PHA, Phytohemagglutinin; TT, tetanus toxoid, n.s., not significant. Journal of Allergy and Clinical Immunology  , DOI: ( /S (95) ) Copyright © 1995 Mosby, Inc. Terms and Conditions

5 FIG. 4 LPT with milk and Damira formula in patients with CMA; results are expressed in cpm. Circles represent milk, and triangles represent Damira formula. Open symbols indicate patients with IgE-mediated symptoms, and solid symbols, patients with non-IgE-mediated symptoms. The median for each group is shown by a bar. Counts per minute are plotted on the y axis on a log scale. Controls and different antigen concentrations are plotted on the x axis. Statistical analysis was performed to compare both proteins at the same concentration. PHA, Phytohemagglutinin; TT, tetanus toxoid, n.s., not significant. Journal of Allergy and Clinical Immunology  , DOI: ( /S (95) ) Copyright © 1995 Mosby, Inc. Terms and Conditions

6 FIG. 5 LPT with casein and hydrolyzed casein in patients with CMA; results are expressed in cpm. Circles represent casein, and triangles represent hydrolyzed casein. Open symbols indicate patients with IgE-mediated symptoms, and solid symbols, patients with non-IgE-mediated symptoms. The median for each group is shown by a bar. Counts per minute are plotted on the y axis on a log scale. Controls and different antigen concentrations are plotted on the x axis. Statistical analysis was performed to compare both antigens at the same concentration. PHA, Phytohemagglutinin; TT, tetanus toxoid, n.s., not significant. Journal of Allergy and Clinical Immunology  , DOI: ( /S (95) ) Copyright © 1995 Mosby, Inc. Terms and Conditions

7 FIG. 6 LPT with whey protein and hydrolyzed whey protein in patients with CMA; results are expressed in cpm. Circles represent whey protein, and triangles represent hydrolyzed whey protein. Open symbols indicate patients with IgE-mediated symptoms, and solid symbols, patients with non-IgE-mediated symptoms. The median for each group is shown by a bar. Counts per minute are plotted on the y axis on a log scale. Controls and antigen concentrations are plotted on the x axis. Statistical analysis was performed to compare both proteins at the same concentration. PHA, Phytohemagglutinin; TT, tetanus toxoid, n.s., not significant. Journal of Allergy and Clinical Immunology  , DOI: ( /S (95) ) Copyright © 1995 Mosby, Inc. Terms and Conditions

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