1. Na,K-ATPase displays distinct polarized localization in different tissues and developmental stages.
Na,K-ATPase displays distinct polarized localization in different tissues and developmental stages. (A–C) Immunolocalization of sodium pump α-subunit in murine choroid plexus (A), developing kidney (B), and adult kidney (C). Arrowheads indicate apical localization of α-subunit. Arrows indicate basolateral localization of α-subunit. (A) Sodium pump α-subunit localizes to the apical membrane of choroid plexus cells. (B) In developing kidneys harvested from embryonic day 17 mice, sodium pump α-subunit localizes to the apical membrane of the epithelial cells of the renal vesicles, which are the earliest structures in nephrogenesis. In newly developing collecting ducts, sodium pump α-subunit localizes to both apical and basolateral membranes or—in more mature collecting ducts—to basolateral membranes only. (C) Sodium pump α-subunit localizes to the basolateral infoldings of adult kidney tubules. (D–F) Schematic views of sodium pump localization in choroid plexus (D), the developing kidney (E), and the adult kidney (F). Sodium pump α-subunit is depicted in red. Bar=10 μm.
Emily H. Stoops, and Michael J. Caplan JASN 2014;25:
©2014 by American Society of Nephrology