1. Coexistence of Two Functioning T-Cell Repertoires in Healthy Ex-Thalassemics Bearing a Persistent Mixed Chimerism Years After Bone Marrow Transplantation
by Manuela Battaglia, Marco Andreani, Marisa Manna, Sonia Nesci, Paola Tonucci, Barbara Persini, Gioacchino Robustelli della Cuna, Arcangelo Nocera, Jack Gorski, Guido Lucarelli, and Raffaele De Palma
Blood
Volume 94(10):
November 15, 1999
©1999 by American Society of Hematology

2. Spectratype analysis of 1 representative donor and recipient pair (UPN688).
Spectratype analysis of 1 representative donor and recipient pair (UPN688). The donor data are from a pretransplant PBMC sample. The recipient data are from PBMC obtained 7 years posttransplant. The histograms show the TCR profile of 9 different BV families (indicated on top of each pair of spectratypes). The donor or recipient origin of each spectratype is identified on the right.
Manuela Battaglia et al. Blood 1999;94:
©1999 by American Society of Hematology

3. SSCP analysis of predominant spectratype bands.
SSCP analysis of predominant spectratype bands. Each pair of lanes represents data derived from different samples. Lanes 1 and 2, BV2 from UPN688; lanes 3 and 4, BV20 from UPN855; and lanes 7 and 8, BV5.3 from UPN1074. Lanes 5 and 6 show results from the analysis of a normal, polyclonal spectratype band. In each case, the first lane represents PCR in which twice the amount of cDNA was used.
Manuela Battaglia et al. Blood 1999;94:
©1999 by American Society of Hematology

4. Spectratype analysis of in vitro culture with PHA
Spectratype analysis of in vitro culture with PHA. PBMCs from UPN688 (A), UPN885 (B), and UPN1074 (C).
Spectratype analysis of in vitro culture with PHA. PBMCs from UPN688 (A), UPN885 (B), and UPN1074 (C). For each patient, the TCR profiles of 3 representative BV families (indicated on top of each pair of spectratypes) are shown. The spectratype of the PBMC before culture (pre) is shown at the top of each panel and that after the culture (post) is shown on the bottom.
Manuela Battaglia et al. Blood 1999;94:
©1999 by American Society of Hematology

5. Proliferative analysis of the in vitro response to different antigens
Proliferative analysis of the in vitro response to different antigens. [3H]thymidine uptake, expressed in cpm, of PBMCs of UPN 688, UPN855, and UPN1074 after in vitro culture with PPD, TT, PHA, and an unrelated third-party alloantigen (see bar legend) is sh...
Proliferative analysis of the in vitro response to different antigens. [3H]thymidine uptake, expressed in cpm, of PBMCs of UPN 688, UPN855, and UPN1074 after in vitro culture with PPD, TT, PHA, and an unrelated third-party alloantigen (see bar legend) is shown. The same assay was made using a normal donor (ND) as a control. One representative experiment of 3 performed is shown.
Manuela Battaglia et al. Blood 1999;94:
©1999 by American Society of Hematology

6. Detection of donor and host cells after in vitro responses to different stimuli.
Detection of donor and host cells after in vitro responses to different stimuli. (A) A representative FISH analysis performed on PBMCs of UPN688 after culture with PPD. The arrow indicates the biotinylated pY3.4 probe complementary to a part of Y chromosome, showing the presence of some recipient cells. (B) RFLP-VNTR analysis performed on PBMCs of the donor (D) and the respective recipient UPN855 (H) pretransplant, on PBMCs of the host after culture with cells of a third party (A), and on the PBMCs of the same transplanted recipient after in vitro culture with TT (T), PPD (P), and PHA (Ph). The top arrow indicates the presence of recipient cells and the bottom arrow indicated the presence of donor cells. (C) Data summary in which the y-axis shows the percentage of donor cells evaluated by FISH or RFLP-VNTR and the x-axis shows the different samples analyzed before (PBMC) and after (PHA, PPD, TT, and Allo) in vitro culture. Different color bars refer to the 3 different patients (see legend).
Manuela Battaglia et al. Blood 1999;94:
©1999 by American Society of Hematology