1. Transcription

35. Function of 5´cap Protection from degradation
Increased translational efficiency
Transport to cytoplasm
Splicing of first exon

37. RNA polymerase II does not usually terminate at distinct site
Pre-mRNA is cleaved ~20 nucleotides downstream of polyadenylation signal (AAUAAA)
~250 AMPs are then added to the 3´ end
Almost all mRNAs have poly(A) tail

38. Function of poly(A) tail
Increased mRNA stability
Increased translational efficiency
Splicing of last intron
AAAAAA