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Volume 3, Issue 3, Pages (May 2010)

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1 Volume 3, Issue 3, Pages 603-609 (May 2010)
Plasticity of Xyloglucan Composition in Bean (Phaseolus vulgaris)-Cultured Cells during Habituation and Dehabituation to Lethal Concentrations of Dichlobenil  Alonso-Simón A. , Neumetzler L. , García-Angulo P. , Encina A.E. , Acebes J.L. , Álvarez J.M. , Hayashi T.   Molecular Plant  Volume 3, Issue 3, Pages (May 2010) DOI: /mp/ssq011 Copyright © 2010 The Authors. All rights reserved. Terms and Conditions

2 Figure 1 Sepharose CL-6B Gel Filtration of Xyloglucan Obtained from Non-Habituated Cells (○),12 μM DCB-Habituated Cells (•), and Dehabituated Cells (•). Markers: blue dextran (Vo), 267, 72.6, and 8.8 kDa dextrans, and glucose. Molecular Plant 2010 3, DOI: ( /mp/ssq011) Copyright © 2010 The Authors. All rights reserved. Terms and Conditions

3 Figure 2 Relative Abundance of Major Xyloglucan Oligosaccharides after XEG-Digestion of Xyloglucan Obtained from Non-Habituated Cells (□), 12 μM DCB-Habituated Cells (▪), and Dehabituated Cells (▪), Representing 87, 86, and 88% of Total Ions Observed, Respectively, in Each Kind of Cell. The bars represent the average ± SD of three measurements. * Significant difference with respect to non-habituated cells as evaluated by Student's t-test (P < 0.05), n = 3. Molecular Plant 2010 3, DOI: ( /mp/ssq011) Copyright © 2010 The Authors. All rights reserved. Terms and Conditions

4 Figure 3 Relative Abundance of Minor Xyloglucan Oligosaccharides after XEG-Digestion of Xyloglucan Obtained from Non-Habituated Cells (□), 12 μM DCB-Habituated Cells (▪), and Dehabituated Cells (▪), Representing 12, 12, and 8% of Total Ions Observed, Respectively, in Each Kind of Cell. The bars represent the average ± SD of three measurements. * Significant difference with respect to non-habituated cells as evaluated by Student's t-test (P < 0.05), n = 3. Molecular Plant 2010 3, DOI: ( /mp/ssq011) Copyright © 2010 The Authors. All rights reserved. Terms and Conditions

5 Figure 4 MALDI–TOF Mass Spectra of Xyloglucan Fragment Oligosaccharides Obtained from Non-Habituated (NH, Top), 12 μM DCB-Habituated (H12, Middle), and Dehabituated Bean Cells (DH, Bottom) Subjected to Total Digestion by XEG (Left) or to Partial (Middle) or Complete (Right) Digestion by Endocellulase. Arrowheads point to cello-oligosaccharides. Molecular Plant 2010 3, DOI: ( /mp/ssq011) Copyright © 2010 The Authors. All rights reserved. Terms and Conditions

6 Figure 5 Proposed Structures of Xyloglucans Obtained from (A) Non-Habituated, (B) 12 μM DCB-Habituated, and (C) Dehabituated Bean Cells. The figure shows the amount of the main oligosaccharides in xyloglucan, considering the percentage of them present in each molecule and the average molecular mass of xyloglucan in each kind of cells, not the exact order of oligosaccharides in the molecule. Molecular Plant 2010 3, DOI: ( /mp/ssq011) Copyright © 2010 The Authors. All rights reserved. Terms and Conditions


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