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Volume 43, Issue 4, Pages 342-350 (April 2003)
Molecular Staging of Prostatic Cancer with RT-PCR Assay for Prostate-Specific Antigen in Peripheral Blood and Lymph Nodes: Comparison with Standard Histological Staging and Immunohistochemical Assessment of Occult Regional Lymph Node Metastases Luis Martı́nez-Piñeiro, Emilio Rios, Montserrat Martı́nez-Gomariz, Teresa Pastor, Mónica de Cabo, Marı́a L. Picazo, José Palacios, Rosario Perona European Urology Volume 43, Issue 4, Pages (April 2003) DOI: /S (03)
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Fig. 1 Sensitivity of RT-PCR in detecting PSA mRNA. (A) Ethydium bromide-stained gel depicting PCR detection of PSA mRNA in LNCaP cells diluted with Jurkat cells. The band is a 355bp signal clearly seen in dilutions up to 10−6. (B) Ethydium bromide-stained gel depicting PCR of PSA in lymph nodes from two control patients (controls) and two positive samples of one patient with pathological confirmed lymph node metastases (positive sample). The 486bp band of PSA DNA derives from genomic DNA and the 355bp band of PSA cDNA derives from messenger RNA (L.O.: left obturator lymph nodes; R.O.: right obturator lymph nodes). European Urology , DOI: ( /S (03) )
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European Urology 2003 43, 342-350DOI: (10.1016/S0302-2838(03)00055-1)
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