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Ovarian minimal residual disease in chronic myeloid leukaemia

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Presentation on theme: "Ovarian minimal residual disease in chronic myeloid leukaemia"— Presentation transcript:

1 Ovarian minimal residual disease in chronic myeloid leukaemia
Ronit Abir, Adina Aviram, Meora Feinmesser, Jerry Stein, Isaac Yaniv, Doris Parnes, Avi Ben-Haroush, Dror Meirow, Esther Rabizadeh, Benjamin Fisch  Reproductive BioMedicine Online  Volume 28, Issue 2, Pages (February 2014) DOI: /j.rbmo Copyright © 2013 Reproductive Healthcare Ltd. Terms and Conditions

2 Figure 1 Quantitative reverse-transcription PCR for ABL (A) and BCR-ABL (B) detection. Typical fluorescence curves obtained for standards (dotted lines) and sample (unbroken lines). Reproductive BioMedicine Online  , DOI: ( /j.rbmo ) Copyright © 2013 Reproductive Healthcare Ltd. Terms and Conditions

3 Figure 2 Two-step nested quantitative reverse-transcription PCR. PCR products were analysed on a 2% agarose gel. Sample lanes 1–4=cryopreserved ovarian tissue sample with a PCR product of 458bp, typical for b3a2 transcripts (the breakpoint of BCR gene is downstream of exon 14); NEG=Philadelphia-negative sample derived from healthy control; NTC=no-template control; POS=Philadelphia-positive sample derived from a CML patient with a PCR product of 383bp, typical for b2a2 transcripts (the breakpoint of BCR gene is downstream of exon 13); ABL=positive control for the gene ABL, demonstrating RNA integrity of the sample); Marker=100-bp DNA size ladder. Reproductive BioMedicine Online  , DOI: ( /j.rbmo ) Copyright © 2013 Reproductive Healthcare Ltd. Terms and Conditions


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