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Novel rapid PCR for the detection of Ile491Phe rpoB mutation of Mycobacterium tuberculosis, a rifampicin-resistance-conferring mutation undetected by.

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Presentation on theme: "Novel rapid PCR for the detection of Ile491Phe rpoB mutation of Mycobacterium tuberculosis, a rifampicin-resistance-conferring mutation undetected by."— Presentation transcript:

1 Novel rapid PCR for the detection of Ile491Phe rpoB mutation of Mycobacterium tuberculosis, a rifampicin-resistance-conferring mutation undetected by commercial assays  E. André, L. Goeminne, A. Colmant, P. Beckert, S. Niemann, M. Delmee  Clinical Microbiology and Infection  Volume 23, Issue 4, Pages 267.e5-267.e7 (April 2017) DOI: /j.cmi Copyright © 2016 The Author(s) Terms and Conditions

2 Fig. 1 (a) Design of the multiplex allele-specific PCR for the detection of the Ile491Phe rpoB rifampicin-resistance mutation. The rpoB_I491_F primer is specific to the wild-type profile, whereas the rpoB_F and rpoB_R primers are universal. (b) Results: the method allowed the correct identification of the rpoB Ile491Phe mutation among the 39/78 rifampicin-resistant strains. Clinical Microbiology and Infection  , 267.e5-267.e7DOI: ( /j.cmi ) Copyright © 2016 The Author(s) Terms and Conditions


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