1. A model Notch response element detects Suppressor of Hairless–dependent molecular switch 
Marc Furriols, Sarah Bray 
Current Biology 
Volume 11, Issue 1, Pages (January 2001)
DOI: /S (00)

2. Figure 1
Su(H)m8 sites confer both activation and repression in vivo. The organization of the different constructs is depicted in (a). The backbone was the minimal promoter from the hsp70 promoter (orange) driving expression of lacZ. The sequences inserted upstream were Gbe (blue), Su(H)m8 (green), a combination of Gbe+ Su(H)m8, and a combination Gbe+ Su(H)MUT in which the Su(H) sites had been mutated (indicated by X). Expression from the different transgenes is shown below [Gbe, (b–d); Su(H)m8, (e–g); Gbe+ Su(H)m8, (h–j); Gbe+ Su(H)MUT, (k–m)] in wing imaginal discs (b,e,h,k), epidermis (c,f,i,l), and trachea (d,g,j,m). All tissues in this and the following figures are from late third instar larvae
Current Biology  , 60-64DOI: ( /S (00) )

3. Figure 2
Gbe+ Su(H)m8 confers an E(spl)mβ-like expression pattern and requires Su(H) and Notch activities. The expression of E(spl)mβ [(a,c); mβ1.5-lacZ] in wing imaginal discs (a) and trachea (c) is largely recapitulated by Gbe+ Su(H)m8(b,d). Decreased Notch activity [(e); Nts1/N55e11 after 1 day at the nonpermissive temperature) eliminates much of the wing disc expression from Gbe+ Su(H)m8; only weak d/v boundary expression remains. Similar loss of activation is seen in clones of cells that lack Su(H)(f,g); absence of green GFP staining indicates Su(H)SF8 mutant cells (e.g., arrows) that correspond to positions where Gbe+ Su(H)m8 (red) expression is reduced [(g) shows red channel only]
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4. Figure 3
Repression is dependent on Su(H) and can be alleviated by Nicd. Expression of Gbe+ Su(H)m8 [depicted in (a) with symbols as in Figure1a) in wild-type [(b,c); wt] Su(H) [(d,e); Su(H)SF8/Su(H)AR9] and Notch [(f,g); Nts/N55e11, 72 hr at 30°C). There is derepression of the transgene in Su(H) mutant tissues but not in Notch mutants. Transient expression of an activated from of Notch [(j,k); hsNicd, 37°C] alleviates repression [compare to wild-type under the same heat shock conditions, (h,i); wt, 37°C]
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5. Figure 4
Model for Notch target gene regulation. In the absence of Notch, DNA-bound Su(H) (green) prevents activators, such as Grainyhead (blue), from promoting transcription. This is likely to be an indirect effect mediated by corepressors (gray) that are recruited by Su(H) and may act by local modification of chromatin [1, 20]. Nicd (orange) is able to alleviate the repression and Su(H)/Nicd is able to cooperate synergistically with transactivators (e.g., Grainyhead, blue), probably via the recruitment of additional cofactors, to promote transcription [25]
Current Biology  , 60-64DOI: ( /S (00) )