1. Detection of Trisomy 12 and Rb-Deletion in CD34+ Cells of Patients With B-Cell Chronic Lymphocytic Leukemia
by B. Gahn, C. Schäfer, J. Neef, C. Troff, M. Feuring-Buske, W. Hiddemann, and B. Wörmann
Blood
Volume 89(12):
June 15, 1997
©1997 by American Society of Hematology

2. Representative flow cytometric analysis of patient no. 1.
Representative flow cytometric analysis of patient no. 1. Ten thousand cells were aquired in listmode on a FACScan with two gates showing the sorted two different maturational subpopulations: CD34+/38+ cells in fluorescence gate R2 with light scatter gate R1 and CD34+/38− cells in fluorescence gate R3 with light scatter gate R1.
B. Gahn et al. Blood 1997;89:
©1997 by American Society of Hematology

3. The percentage of cells within sorted CD34+/38+ (□) and CD34+/38− () fractions that contain the genetic marker detected by FISH. (▪) The percentage of sort unpurity.
The percentage of cells within sorted CD34+/38+ (□) and CD34+/38− () fractions that contain the genetic marker detected by FISH. (▪) The percentage of sort unpurity.
B. Gahn et al. Blood 1997;89:
©1997 by American Society of Hematology

4. Unsorted peripheral blood cells after FISH with a chromosome 12-specific α satellite DNA probe conjugated to spectrum orange.
Unsorted peripheral blood cells after FISH with a chromosome 12-specific α satellite DNA probe conjugated to spectrum orange. Three fluorescent hybridization signals indicate trisomy 12; two signals represent diploid cells (left). The right side shows a sorted CD34+/38+ cell hybridized to a spectrum green labeled a chromosome 12-specific α satellite DNA probe. The three hybridization spots indicate trisomy 12 (right).
B. Gahn et al. Blood 1997;89:
©1997 by American Society of Hematology

5. Flow cytometric analysis of bone marrow of a CLL patient stained with CD34 FITC (HPCA-2), CD20 PerCP (L27), and CD5 PE (L17F12).
Flow cytometric analysis of bone marrow of a CLL patient stained with CD34 FITC (HPCA-2), CD20 PerCP (L27), and CD5 PE (L17F12). Fifty thousand cells were aquired in listmode on a FACScan. The leukemic cells are depicted blue by simultaneous analysis of CD5 PE and CD20 PerCP, whereas the CD34+ cells appear as green. There is no population detectable coexpressing CD34, CD5, and CD20.
B. Gahn et al. Blood 1997;89:
©1997 by American Society of Hematology