1. Volume 125, Issue 5, Pages 1379-1387 (November 2003)
Distinct costimulation dependent and independent autoreactive T-cell clones in primary biliary cirrhosis 
Takashi Kamihira, Shinji Shimoda, Kenichi Harada, Akira Kawano, Mizuki Handa, Eishi Baba, Koichi Tsuneyama, Minoru Nakamura, Hiromi Ishibashi, Yasuni Nakanuma, M.Eric Gershwin, Mine Harada 
Gastroenterology 
Volume 125, Issue 5, Pages (November 2003)
DOI: /j.gastro

2. Figure 1
T-cell-reactive precursor frequency to PDC-E2 163–176 (10−7) using irradiated autologous PBMC (A) or mitomycin C-treated L-DR5 (B) as APC. There is a significant increase in precursor frequency of PBMC from patients with PBC compared with controls (P < .01). Y axis indicates the cell numbers of PDC-E2 163–176-reactive T cells/107 PBMC.
Gastroenterology  , DOI: ( /j.gastro )

3. Figure 2
Proliferation assays (CPM) of T-cell clones that respond to PDC-E2 163–176. There are distinct differences in response when (A) costimulation-dependent clones are compared with (B) costimulation-independent type clones, particularly when L-DR53 cells are used as APCs (P < 0.001). Y axis indicates the incorporation of 3H-TdR.
Gastroenterology  , DOI: ( /j.gastro )

4. Figure 3
The effect of anti-B7-1 or B7-2 antibody effect on T-cell proliferation assay (CPM) of (A) costimulation-dependent type (MN29) or (B) independent type (HT7); mAbs suppress the proliferation of only costimulation-dependent clones (P < 0.001). Y axis indicates the incorporation of 3H-TdR.
Gastroenterology  , DOI: ( /j.gastro )

5. Figure 4
Flow cytometric analysis of CD4 and CD28 of PBMC from patients with PBC, healthy controls, and patients with HCV-induced chronic hepatitis. There are significantly more CD4+ CD28− peripheral T cells in early stage compared with late-stage PBC, healthy controls, or patients with chronic hepatitis (P < 0.01). Y axis indicates the percentage of CD4+ CD28− T cells in the total CD4+ T cells.
Gastroenterology  , DOI: ( /j.gastro )

6. Figure 5
Flow cytometric analysis (A) of CD4 and CD28 or (B) immunohistochemistry of liver-infiltrated mononuclear cells from patients with PBC and patients with cirrhosis infected with HCV who had liver transplantation. In both A and B, there are more CD4+ CD28− liver-infiltrating T cells in patients with PBC (P < 0.001). Y axis indicates the percentage of CD4+ CD28− T cells in the total CD4+ T cells.
Gastroenterology  , DOI: ( /j.gastro )

7. Figure 6
Characteristics of costimulation-dependent and costimulation-independent clones. Note the contrast in the inhibitory effect of BEC on the proliferation of T-cell clone MM29 compared with HT7 in their response to PDC-E2 163–176 (see A vs. D). Also, note that BECs, as APCs, suppress the proliferation of both costimulation-dependent and independent T-cell clones (again contrast A and D). The presence of either BEC or the supernatant of BEC suppresses the proliferation of both costimulation-dependent and -independent T-cell clones stimulated with PDC-E2 163–176-pulsed irradiated autologous PBMC. The effector target ratio for the response of the T-cell clones MM29 and HT7 in the presence or absence of PDC-E2 163–176 is shown in B and E. Note that both T-cell costimulation-dependent and -independent clones had cytotoxic activity against antigen-pulsed BECs. Finally, the anergic characteristics of clones MM29 and HT7 are shown in C and F. Costimulation-dependent, but not -independent T-cell clones, become anergic after coculture with antigen-pulsed BECs. Y axis indicates the incorporation of 3H-TdR.
Gastroenterology  , DOI: ( /j.gastro )