1. Bio 9B: Monday, 11.30.09 Title: Movement Across Cell Membranes – Day 1
Homework:
Read pages (Section 7-3): Take notes based on the questions on the back of Assignment Sheet and write your answers in your notebook.
Do Now: (in your notebook)
Which molecule is bigger, starch or glucose? Explain with a diagram in our notebook.
Today’s Objectives:
Design an experiment by drawing from your knowledge of starch, glucose, cell membranes, and experimental indicators that test for the presence of starch and glucose.

2. Movement Across cell Membranes
Moretti and Dickson

3. Diffusion Across Cell Membranes
Designing a Lab:
Diffusion Across Cell Membranes
Planning the Experiment:
Individual:
Read Overview
Small Groups:
Planning the Experiment
How do we test for Starch and Glucose?
Whole Class Discussion
Small Group:
Experimental Set-Up
Whole Class Review:
Model Cell w/ Dialysis Tubing Membrane
Starch & Glucose Solution
Water
Objectives for Class:
Design an experiment by drawing from your knowledge of starch, glucose, cell membranes, and experimental indicators that test for the presence of starch and glucose.

4. Diffusion Across Cell Membranes
Designing a Lab:
Diffusion Across Cell Membranes
Class Discussion:
Starch
How do we test for it?
Use Iodine
How do we know starch has passed through membrane?
Iodine outside the dialysis bag turns from yellow-orange to purplish- brown
Glucose
Boil it in Benedicts solution or use a glucose test strip
How do we know glucose has passed through the membrane?
Glucose test strip will change color
Objectives for Class:
Design an experiment by drawing from your knowledge of starch, glucose, cell membranes, and experimental indicators that test for the presence of starch and glucose.

5. Bio 9B: Tuesday, 12.1.09 Title: Movement Across Cell Membranes – Day 2
Homework:
Type the following sections of the Diffusion Lab:
Background Info
Purpose/Hypothesis
Summary of Procedure
Do Now: (in your notebook)
What is happening to the dye in the water? Based on your reading, what is this called?
Today’s Objectives:
Design an experiment to test diffusion of starch and glucose across a membrane
Use scientific vocabulary to explain how a cell membrane controls what enters and leaves a cell

6. Diffusion Across Cell Membranes
Designing a Lab:
Diffusion Across Cell Membranes
Review Experimental Set-Up:
Part I:
Set-up Dialysis Bag
Moisten tube and tie one end
Add the starch & glucose solution to the tube
Tie the top of the tube
Rinse w/ tap water to wash excess solution from outside of bag
Set-up Beaker
Fill a 250 ml beaker ¾ of the way with water
Add Iodine to water – mixture should be orange-ish
Put dialysis bag into beaker with water and iodine
Part II: Wait 30 min to allow for substances to move across the membrane
Starch & Glucose Solution
Water and Iodine
Objectives for Class:
Design an experiment by drawing from your knowledge of starch, glucose, cell membranes, and experimental indicators that test for the presence of starch and glucose.

7. Diffusion Across Cell Membranes
Designing a Lab:
Diffusion Across Cell Membranes
Review Experimental Set-Up:
Part III:
How do you know the following substances have left the bag:
Starch?
Glucose?
What other substances might move across the bag? How will you know?
Starch & Glucose Solution
Water and Iodine
Objectives for Class:
Design an experiment by drawing from your knowledge of starch, glucose, cell membranes, and experimental indicators that test for the presence of starch and glucose.

8. New Vocabulary Lipid Bilayer
Two layers of lipids that form the outer membrane of a cell.
Flexible
Strong
Do not Dissolve in water
zoom out
Image to the right shows the lipid bilayer of a cell. Notice how it has other components like protein channels & “pumps” built into it.
Objectives for Class:
Use scientific vocabulary to explain how a cell membrane controls what enters and leaves a cell

9. New Vocabulary Selective Permeability
When a cell membrane (or any membrane for that matter) only allows certain materials to pass across the membrane and not others
Substance usually pass across based on the size, shape, and charge (+/-/neutral) of the molecules
Objectives for Class:
Use scientific vocabulary to explain how a cell membrane controls what enters and leaves a cell

10. New Vocabulary Concentration Diffusion Osmosis
How much “stuff” is in a given amount of liquid
Mass of a solute (stuff in the liquid) per a given volume of the total solution
i.e.: 12g of salt in 3L of water = 12g/3L = 4g/L
Diffusion
Movement of molecules from a
high concentration (more stuff) to low concentration (less stuff)
Osmosis
Movement of water through a selectively permeable membrane from a
high concentration to low concentration
Note: For osmosis to occur, the membrane only allows water to pass across and not the solute
Osmosis
Objectives for Class:
Use scientific vocabulary to explain how a cell membrane controls what enters and leaves a cell

11. New Vocabulary
Diffusion, Osmosis and Selective Permeability In Action:
Objectives for Class:
Use scientific vocabulary to explain how a cell membrane controls what enters and leaves a cell

12. New Seats Jisrael Danika Weston Loic Nick Wilson Emma Chyneree
Yikaalo Jeff
Graceann Tina
Reggie Josh
Sophia Rachelle
Gaby Christina
Andrew
Djinnie Mildred
Ruth Scarlett
Adel Jennifer

13. Bio 9B: Thursday, 12.3.09 Title: Movement Across Cell Membranes – Day 3
Homework:
Type the following sections of the Diffusion Lab:
Data/ Results
Analysis
Conclusion
Bring in a printed copy of your background information section to class tomorrow!!!!
Do Now:
Clear your desks except for lab notebook and the Diffusion Lab Set-up Sheet.
Today’s Objectives:
Implement an experiment to test diffusion of starch and glucose across a membrane
Use scientific vocabulary to explain how a cell membrane controls what enters and leaves a cell
Double Block

14. Diffusion Across Cell Membranes
Implementing the Lab:
Diffusion Across Cell Membranes
Review Experimental Set-Up:
Part I:
Preparing Dialysis Bag
Soak the dialysis tube in water to soften
Tie one end into a knot
Fill the tube about half-way w/ starch/ glucose solution
Tie the other end of the tube into a knot to seal the bag
Rinse w/ tap water to wash excess solution from outside of bag
Preparing the Beaker
Label your beaker
Fill a 140 ml beaker ¾ of the way with water
Add drops of Iodine to water and mix . Note the color of the water
Test water with glucose test strip and note any color change
Preparing the Model:
Put dialysis bag into beaker with water and iodine solution
Part II:
Wait 30 min to allow for substances to move across the membrane
Part III:
Data Collection
Diagram the experimental set-up
Create a data table to collect data in your notebooks
Collect data by recording any color change to the beaker, dialysis bag, and/or glucose test strip. (note: we will finish out notes before you record the data into your table) z
Starch & Glucose Solution
Water and Iodine
Objectives for Class:
Implement an experiment to test diffusion of starch and glucose across a membrane

15. New Vocabulary Facilitated Diffusion
When a substance diffuses through a membrane by going through a protein channel
Regular diffusion goes through the
lipid bilayer
Protein Channel
Objectives for Class:
Use scientific vocabulary to explain how a cell membrane controls what enters and leaves a cell

16. New Vocabulary Active Transport
When a substance is pumped across a membrane from where there’s a low concentration (less stuff) to where there’s a high concentration (more stuff)
Requires a pump (made of protein) and ATP energy!
Objectives for Class:
Use scientific vocabulary to explain how a cell membrane controls what enters and leaves a cell

17. Diffusion Across Cell Membranes
Implementing the Lab:
Diffusion Across Cell Membranes
Review Experimental Set-Up:
Part I:
Preparing Dialysis Bag
Soak the dialysis tube in water to soften
Tie one end into a knot
Fill the tube about half-way w/ starch/ glucose solution
Tie the other end of the tube into a knot to seal the bag
Rinse w/ tap water to wash excess solution from outside of bag
Preparing the Beaker
Label your beaker
Fill a 140 ml beaker ¾ of the way with water
Add drops of Iodine to water and mix . Note the color of the water
Test water with glucose test strip and note any color change
Preparing the Model:
Put dialysis bag into beaker with water and iodine solution
Part II:
Wait 30 min to allow for substances to move across the membrane
Part III:
Data Collection
Diagram the experimental set-up
Create a data table to collect data
Collect data by recording any color change to the beaker, dialysis bag, and/or glucose test strip.
Starch & Glucose Solution
Water and Iodine
Objectives for Class:
Implement an experiment to test diffusion of starch and glucose across a membrane

18. Background Information Brainstorm
Implementing the Lab:
Diffusion Across Cell Membranes
Background Information Brainstorm
Cell membrane – lipid bilayer
Selective Permeability
Dialysis tubing (why did we use it, what does it do, characteristics of it)
Diffusion
Concentration
Glucose and starch (what are they, what they do, how big they are, how to test for glucose and starch)
Iodine for starch and test strip for glucose, indicators and why they are called that
Osmosis (optional)
Objectives for Class:
Implement an experiment to test diffusion of starch and glucose across a membrane

19. Bio 9B: Friday, 12.4.09 Title: Movement Across Cell Membranes – Day 4
Homework:
Typed Final Draft of the Diffusion Lab Due Monday at the start of Class
Do Now:
Take out the lab report you are writing. Which molecules moved across the dialysis bag membrane? Why?
Today’s Objectives:
Use scientific vocabulary to explain how a cell membrane controls what enters and leaves a cell
Make clear connections between various vocabulary words and concepts to each other and to the purpose of the lab, so as to write a better Background Information Section.

20. New Vocabulary
Diffusion, Osmosis and Selective Permeability In Action:
Objectives for Class:
Use scientific vocabulary to explain how a cell membrane controls what enters and leaves a cell

21. Diffusion Across Cell Membranes
Wrapping up the Lab:
Diffusion Across Cell Membranes
Writing Better Background Information Sections
Task I:
Read the to example Background Information sections from the Denatured Enzyme Lab and grade them according to the rubric.
Objectives for Class:
Use scientific vocabulary to explain how a cell membrane controls what enters and leaves a cell
Make clear connections between various vocabulary words and concepts to each other and to the purpose of the lab, so as to write a better Background Information Section.

22. Diffusion Across Cell Membranes
Wrapping up the Lab:
Diffusion Across Cell Membranes
Writing Better Background Information Sections
Task II:
Create a mind-map to connect the vocabulary and concepts for the Background Information Section. This will help us outline this section for us as well.
Objectives for Class:
Use scientific vocabulary to explain how a cell membrane controls what enters and leaves a cell
Make clear connections between various vocabulary words and concepts to each other and to the purpose of the lab, so as to write a better Background Information Section.

23. Background Information Brainstorm
Wrapping up the Lab:
Diffusion Across Cell Membranes
Background Information Brainstorm
Cell membrane – lipid bilayer
Selective Permeability
Dialysis tubing (why did we use it, what does it do, characteristics of it)
Diffusion
Concentration
Glucose and starch (what are they, what they do, how big they are, how to test for glucose and starch)
Iodine for starch and test strip for glucose, indicators and why they are called that
Osmosis (optional)
Objectives for Class:
Use scientific vocabulary to explain how a cell membrane controls what enters and leaves a cell
Make clear connections between various vocabulary words and concepts to each other and to the purpose of the lab, so as to write a better Background Information Section.

24. Diffusion Across Cell Membranes
Wrapping up the Lab:
Diffusion Across Cell Membranes
Writing Better Background Information Sections
Task III:
Use mind-map/ outline to re-write your background information section more effectively.
Objectives for Class:
Use scientific vocabulary to explain how a cell membrane controls what enters and leaves a cell
Make clear connections between various vocabulary words and concepts to each other and to the purpose of the lab, so as to write a better Background Information Section.

25. Diffusion Across Cell Membranes
Wrapping up the Lab:
Diffusion Across Cell Membranes
Data Analysis:
Work in your groups to explain what your results mean.
If there are color changes, state was this means in terms of what substances are present, but
DO NOT explain why the substance is where it is (this should go in the conclusion section)
Starch & Glucose Solution
Water and Iodine
Objectives for Class:
Use scientific vocabulary to explain how a cell membrane controls what enters and leaves a cell
Make clear connections between various vocabulary words and concepts to each other and to the purpose of the lab, so as to write a better Background Information Section.