1. Ubiquitination of Drp1 by MARCH‐V.
Ubiquitination of Drp1 by MARCH‐V. COS7 cells were transiently transfected with expression plasmids encoding HA–ubiquitin (0.6 μg), 6 × Myc–Drp1 (1.0 μg) and empty vector (lanes 1,4,7), Flag–Mar5 (lanes 2,5,8) or Flag–RINGmut (lanes 3,6,9; 1.4 μg). Whole‐cell lysates were immunoprecipitated (IP) with anti‐Myc beads followed by western blotting (WB) with Myc (top left) or HA antibody (top right). The asterisk indicates ubiquitinated Drp1. The bottom panel shows the result of western blotting of the lysates with Myc or Flag antibody. HA, haemagglutinin.
Nobuhiro Nakamura et al. EMBO Rep. 2006;7:
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