1. smart compact affordable
QIAxtractor
smart compact affordable
Brant Bassam PhD
Andrea Tesoriero
Application Specialist

2. QIAxtractor is a seamless integration to your workflow
post PCR
analysis
real time
PCR or RT PCR
PCR setup
nucleic acid extraction
Sample preparation
Sample preparation
DNA extraction
aliquoting
enzymatic digestion
post
purification QC
enzymatic digestion
PCR setup
PCR &
analysis

3. COMPACT QIAxtractor moves according to the experiment requirements
Fits into any work space and is easily movable
Very small footprint
Light and easily movable (25 kg; 51 lbs)
Vacuum and waste stations located under the bench for more space and convenience
8-channel pipetting head without any tubing for more accuracy and easy maintenance
Comes with a laptop for even more flexibility
8-Channel pipetting head

4. Bind-Elute Protocol Sample loaded with lysis buffer at 1:2 ratio
Lyse for 10 minutes
Load the capture plate and apply vacuum
Wash captured DNA with 1  Lysis Buffer
2  Propanol wash
1  Ethanolic wash
Residual Ethanol is removed by vacuum
Capture plate moves to the elution station
Elution buffer loaded and vacuum applied
DNA elutes into a 96-well cluster tube plate 4 4

5. Why automate? Minimize cross contamination risk Reduce human error
Reduce costs
Increase throughput & save time
Often the bottleneck in a project Work in increments of 8 samples (from 8 to 96) 96 samples in about 96 minutes
Increase safety
Minimum exposure to dangerous chemicals
Versatility and choice
DX liquid or tissue reagent,
Vx reagent
Wide range of protocols for DNA, RNA and viral nucleic acids
Associated plasticware
Maintain your sanity (pain threshold is about 25 extractions per week!) 5 5

6. Sample types successfully extracted
Blood -whole, fresh, frozen, buffycoat,Human, Mouse, Turtle, chickens, sheep Bovine Serum/Plasma, -HIV, Hep B , Hep C, CMV
Urine Chlamydia and N. Gonorrhoea
Swabs HSV1 and 2
Solid Tissue Mouse Tails, Prawn Tissue, Bird Quill Pulp
Nasal Aspirates MPV (a single stranded RNA Virus)
Cultured cells
Micro Organisms Bacteria (Gram Pos. and Gram Neg.) and Fungi
Plants Fruit, bark, leaves and stems
Faeces Norris Virus
Forensics cigarette butts, food scraps, finger prints, hair, fabric, FTA cards 6 6

7. QIAxtractor Easy to use system Low cost possible cost per extraction
Hands-free, walk-away operation
High speed + high throughput (96 well)
Consolidated chemistry minimum reagents and kit types work for all sample types
Complete integrated system solution automation, reagents, consumables, protocols, options 7 7

8. Bench Top Unit 770 mm open 390 mm closed 490 mm 25 kg
Work surface enclosed by transparent cover
8-Channel pipetting head
Dual position Manifold
1. Vacuum to Waste
2. Vacuum to Elute 1 2
Tip discard chute
4 SBS plate positions
3–8 Reagent Tubs (variable volumes)
New photos
New head design
HEPA + UV in photo
Add “compact” size 8 8

9. Under Bench Vacuum/Waste Station
To X-tractor Gene manifold
Vacuum chamber coupling
19 kg
520 mm
495 mm
400 mm
Bottle full sensor
Pump coupling
High power pump
Carbon exhaust filter
Waste bottle 4 L sanitary quick disconnect bottle
PolyCap pump filter
Vacuum sensor
Software regulated
-5 to -70 kPa, 80 L/min 9 9

10. Instrument Options UV Light HEPA filter
Irradiates surfaces between runs
Robot arm moves so light reaches all areas
Software controlled
Timed post-run operation
Must be factory fitted
HEPA filter
Positive pressure clean air supply
Software controlled
Integrated design
Easy filter change
Can be added after install
New photo 10 10

11. CorProtocols™ for different applications
Available for web download
Each comprises:
PDF Protocol (Instructions for Use)
X-tractor Gene Run File 11 11

12. For standard protocols simply:
Easy-to-use Wizard
For standard protocols simply:
1. Click-select the columns to extract (in columns of 8)
2. Press Jump to End button and start run! 12 12

13. Software Intuitive color-coded interface mirrors actual deck layout
Active screen prompts
For each reagent tub position, volumes needed are calculated for the number of samples to be extracted 13 13

14. Barcode Reader Compatible
Sample names and information can also be imported or exported
Update 14 14

15. Pre- and Post-Run Reports
HTML report (universal format)
Provides details of a run
Documentation & Traceability (e.g. sample, batch and lot identifiers)
Can be directly:
Printed
Saved ed
Exported to Word 15 15

16. X-tractor Plasticware Pack (standard)
960 x OnCor™ C 200 µL Tips
5 x Square-well Lysis Blocks
60 x Elution Plate 8-Strip Caps
5 x Capture Plates
5 x Elution Plates (96 plain tube cluster rack)
5 x Sealing Films
P/N 2270 Sufficient for 480 Extractions 16 16

17. OnCor™ C Tips rack design
SBS rack with replaceable lid for easy removal and storage between runs
Total rack enclosure for security and contamination resistance
Fully pocketed rack design
Separate pockets for each tip prevents cross-contamination, allowing tip re-use during a run
Tip re-use reduces costs
Uses only 1.5 tip racks per 96 extractions
P/N 2299 OnCor™ C 200 µL Fine Bore Tip
96 tips/rack, 10 racks/box 17 17

18. QIAxtractor minimizes cross-contamination risk Real-time amplification analysis of all samples
Real-Time Analysis
Real-Time quantitative amplification analysis was done on all samples targeting the G6PD1 gene to single copy sensitivity (40 cycles) to test for inhibitors and for evidence of cross-contamination
Amplification Results
early, reproducible amp. of all samples
no evidence of PCR inhibitors
Cross-contamination Results
no amplification of NTC samples
no evidence of cross-contamination

19. QIAxtractor optimizes nucleic acid extraction yield Application in Veterinary Diagnostics
Viral detection of EHV-1 horses Dr. N. Pusterla at University of California, School of Veterinary Medicine
Comparison of extraction efficiencies and DNA quality between manual and automated DNA extraction methods by real time analysis
QIAxtractor (DX)
Manual method (DNeasy)
DNA concentration from blood aliquots (ug/100ul) [Absorbance at 260nm]
5.56 (+/- 3.98)
2.44 (+/- 1.21)
Cycle threshold (Ct) values [threshold=0.1, baseline 3-15]
21.1 (0.4)
22.2 (0.5)
Real time analysis
evaluation of DNA quality: detection of the universal 18S rRNA housekeeping gene
detection of EHV-1: amplification of the glycoprotein B (gB) gene
Results
significant higher concentrations of DNA recovered after automated extraction (p=0.001)
significant stronger Ct values for the automated solution (p=0.01)

20. Rapid & sensitive detection of large numbers of specimens Application in Medical Diagnostics
Detection of noroviruses in fecal specimens Dr. J.A. Marshall at Victorian Infectious Diseases Reference Laboratory, Victoria (Australia)
Experiment: total RNA extraction from fecal specimens + Analysis by multiplex RT PCR
Manual: QIAamp viral RNA
Manual: Roche high pure viral RNA
Universal + QIAamp Virus Biorobot MDX
QIAxtractor + VX chemistry
Time for 36 specimens 2h -
Time for 96 specimens
2h30
1h30
Time Operator was actually busy
25 min
15 min
Mention:
- that automation solutions save staff time for more engaging tasks
- Universal performs at the same speed as QIAxtractor when same protocol / washing steps
Results
no significant difference between the manual and automation methods
automation methods required less material
automation methods process more samples in less time

21. Benefits Consistency & Workflow
Reduce human error and free-up technician time
Standardisation
Between labs and runs
Documentation
Pre- and Post-Run reports
Safety
No phenol/chloroform
All waste safely contained for disposal
Harmful fumes are carbon filtered out
Work area completely enclosed by hood
Run pauses if the hood is opened 21 21

22. SMART QIAxtractor is as convenient as QIAGEN can be
Fast
96 samples in 96 min
Membrane tolerance: double flow rate; 4x more binding capacity compared to the other plates on the market
Robust
Just 2 chemistries (DX/VX) for NA extraction from virtually any sample type
Consolidated chemistry for “liquids” and “tissues”
Ultra-effective lyses buffer
Easy
Very intuitive and flexible software
Just a few clicks of the mouse are enough to start a run
Safe
Innovative features: enclosed hood, UV light, HEPA filter …
Optimal protection of the extraction and of the operator
Individual elution tubes for easy downstream processing
Re-use of tips results in little waste
Membrane tolerance: double flow rate; 4x more binding capacity
Membrane tolerance: double flow rate, 4x more binding capacity -> compared to what ?

23. Gracias por su atención