1. A B
HT29
HCT15
Colo205
Ls174T
LS180
Isotypic control
concentration (IU/ml/106 cells)
7.6%
0.8%
95.3%
26.3%
42.2%
CA19-9
Cell number
FITC
FUT3
FUT6
HT29
HCT15
Colo205
Ls174T
LS180 C
Isotypic control
Relative expression ( / ß-actin)
50.2%
0.7%
99.83%
57.4%
88.7%
sLex
Cell number
FITC
Supplementary Figure 1. CA19-9 and sLex expression correlated with FUT3 and FUT6

2. Supplementary Figure 2. Suppression of FUT3 and/or FUT6 by siRNAs
siFUT3
+ siFUT6
Colo205
LS174T
No treat
Random
siFUT3
FUT3
1.0
0.94
0.23
0.22
ß-actin
siFUT3
+ siFUT6
No treat
Random
siFUT6
FUT6
1.0
1.02
0.28
0.30
ß-actin
Supplementary Figure 2. Suppression of FUT3 and/or FUT6 by siRNAs

3. A
random
siRNA1
siRNA2
FUT1
1.0
0.26
0.28
FUT2
1.0
0.36
0.32
FUT4
1.0
0.21
0.22
FUT5
1.0
0.44
0.72
FUT7
1.0
0.33
0.43
FUT8
1.0
0.27
0.35 B NT
Random
siFUT1
siFUT2
siFUT4
siFUT5
siFUT7
siFUT8
pSmad2
1.0
1.36
1.0
1.25
1.0
3.17
1.0
1.38
1.0
1.33
1.0
1.42
1.0
1.52
1.0
1.35
Smad2
TGF-β – + – + – + – + – + – + – + – +
Supplementary Figure 3. Suppression of FUT3 and FUT6 inhibits Smad2 phosphorylation upon TGF-ß stimulation

4. TGF-β (hr)
IgG 6 12
TßR-I IP
/ AAL IB
TßR-I
1.00
1.05
1.04
TßR-I IP
/ TßRI IB
TßR-I
Supplementary Figure 4. Fucosylation of TßR-I was not affected by TGF-ß stimulation

5. siFUT3 + 6
Random
siFUT3
siFUT6 NT
TßR-I IP
/ CA19-9 IB
1.00
1.05
0.35
0.25
0.29
siFUT3 + 6
Random
siFUT3
siFUT6 NT
TßR-I IP
/ sLex IB
1.00
0.93
0.34
0.23
0.16
Supplementary Figure 5. TßRs bound-CA19-9 and sLex inhibited by siFUT3 and/or siFUT6