Presentation is loading. Please wait.

Presentation is loading. Please wait.

Innate mechanism of pollen- and cat dander–induced oxidative stress and DNA damage in the airways  Koa Hosoki, MD, PhD, David Redding, MD, Toshiko Itazawa,

Published byErlin Cahyadi Modified over 6 years ago

Similar presentations

Presentation on theme: "Innate mechanism of pollen- and cat dander–induced oxidative stress and DNA damage in the airways  Koa Hosoki, MD, PhD, David Redding, MD, Toshiko Itazawa,"— Presentation transcript:

1 Innate mechanism of pollen- and cat dander–induced oxidative stress and DNA damage in the airways 
Koa Hosoki, MD, PhD, David Redding, MD, Toshiko Itazawa, MD, PhD, Anirban Chakraborty, PhD, Nisha Tapryal, PhD, Sun Qian, PhD, Huibin Qi, PhD, Leopoldo Aguilera-Aguirre, PhD, Allan R. Brasier, PhD, Veeranki Sreenivas Phani, PhD, Tapas K. Hazra, PhD, Istvan Boldogh, PhD, Sanjiv Sur, MD  Journal of Allergy and Clinical Immunology  Volume 140, Issue 5, Pages e5 (November 2017) DOI: /j.jaci Copyright © Terms and Conditions

2 Fig 1 TLR4-dependent intracellular ROS generation. A, Many allergenic extracts induce greater ROS generation in TLR4Hi cells compared with TLR4Null cells (n = 6-11 per group). B, Some allergenic extracts and LPS do not induce TLR4-dependent ROS generation (n = 6-11 per group). C, RWPE and CDE induce a greater increase in GSSG in supernatants from TLR4Hi than TLR4Null cells (n = 5 per group). H2DCF-DA, 2′,7′-Dichlorodihydrofluoresceine diacetate. *P < .05, **P < .01, ***P < .001, and ****P <  NS, Not significant. Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © Terms and Conditions

3 Fig 2 TLR4-dependent oxidative stress and DNA damage induced by allergenic extracts. A and B, Single challenge with RWPE (Fig 2, A) or CDE (Fig 2, B) induces greater levels of GSSG in BALF of WT than Tlr4 knockout mice. In the WT or TLR4 antioxidant group, N-acetyl cysteine and ascorbic acid was administered 1 hour before RWPE challenge (n = 5-8 per group). C, RWPE induces greater reductions in intracellular antioxidant glutathione (iGSH) levels in TLR4Hi than in TLR4Null cells (n = 5 per group). D, RWPE induces greater mitochondrial ROS generation in TLR4Hi than TLR4Null cells (n = 3-4 per group). E, RWPE induces greater comet tail moments in TLR4Hi than TLR4Null cells (n = 50 per group). F, RWPE induces greater damage in the lung genomic DNA from WT compared with Tlr4 knockout mice (n = 5-6 per group). *P < .05, **P < .01, ***P < .001, and ****P <  NS, Not significant. Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © Terms and Conditions

4 Fig E1 Coimmunoprecipitation/western blot analysis. HEK 293 cells stably overexpressing HA-tagged TLR4 but not MD2 (TLR4HA) were transfected with plasmid encoding MD2 to generate TLR4-MD2 expressing cells (TLR4HA-MD2).E6 Stimulation of TLR4Hi-MD2 cells with RWPE induced rapid association of TRAF6 with TLR4 in 10 and 30 minutes. IP, Immunoprecipitation; WB, Western blotting. Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © Terms and Conditions

5 Fig E2 RWPE challenge rapidly increases oxidative DNA damage in the noses of allergic human subjects. Intranasal RWPE challenge in human subjects is shown. Patients with allergic rhinitis and positive skin prick test responses to RWPE were challenged with saline on one day and RWPE on another. A, RWPE challenge increased nasal symptom scores for congestion, drainage, and sneezing recorded on subjective scale scores of 0 to 3, yielding a combined total symptom score for all 3 symptoms of a maximum of 9. B, RWPE challenge increased levels of 8-OHdG in nasal fluids 30 minutes after challenge. *P < .05, **P < .01, and ***P < .001. Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © Terms and Conditions

Download ppt "Innate mechanism of pollen- and cat dander–induced oxidative stress and DNA damage in the airways  Koa Hosoki, MD, PhD, David Redding, MD, Toshiko Itazawa,"

Similar presentations

Nasal challenge with allergen leads to maxillary sinus inflammation

Nasal challenge with allergen leads to maxillary sinus inflammation
Receptor for advanced glycation end products and its ligand high-mobility group box-1 mediate allergic airway sensitization and airway inflammation  Md.

Receptor for advanced glycation end products and its ligand high-mobility group box-1 mediate allergic airway sensitization and airway inflammation  Md.
Effect of 2-year placebo-controlled immunotherapy on airway symptoms and medication in patients with birch pollen allergy  Monica B. Arvidsson, MD, Olle.

Effect of 2-year placebo-controlled immunotherapy on airway symptoms and medication in patients with birch pollen allergy  Monica B. Arvidsson, MD, Olle.
Allergen endotoxins induce T-cell–dependent and non–IgE-mediated nasal hypersensitivity in mice  Naruhito Iwasaki, MD, Kazufumi Matsushita, PhD, Ayumi.

Allergen endotoxins induce T-cell–dependent and non–IgE-mediated nasal hypersensitivity in mice  Naruhito Iwasaki, MD, Kazufumi Matsushita, PhD, Ayumi.
The antimicrobial protein short palate, lung, and nasal epithelium clone 1 (SPLUNC1) is differentially modulated in eosinophilic and noneosinophilic chronic.

The antimicrobial protein short palate, lung, and nasal epithelium clone 1 (SPLUNC1) is differentially modulated in eosinophilic and noneosinophilic chronic.
Weiguo Chen, PhD, Umasundari Sivaprasad, PhD, Aaron M

Weiguo Chen, PhD, Umasundari Sivaprasad, PhD, Aaron M
Robert J. Snelgrove, PhD, Lisa G

Robert J. Snelgrove, PhD, Lisa G
Fluticasone furoate nasal spray reduces the nasal-ocular reflex: A mechanism for the efficacy of topical steroids in controlling allergic eye symptoms 

Fluticasone furoate nasal spray reduces the nasal-ocular reflex: A mechanism for the efficacy of topical steroids in controlling allergic eye symptoms 
Cigarette smoke extract induces thymic stromal lymphopoietin expression, leading to TH2-type immune responses and airway inflammation  Yuki Nakamura,

Cigarette smoke extract induces thymic stromal lymphopoietin expression, leading to TH2-type immune responses and airway inflammation  Yuki Nakamura,
Distinct TLR-mediated pathways regulate house dust mite–induced allergic disease in the upper and lower airways  Ji-Hwan Ryu, PhD, Jung-Yeon Yoo, BS,

Distinct TLR-mediated pathways regulate house dust mite–induced allergic disease in the upper and lower airways  Ji-Hwan Ryu, PhD, Jung-Yeon Yoo, BS,
Innate IL-13–producing nuocytes arise during allergic lung inflammation and contribute to airways hyperreactivity  Jillian L. Barlow, PhD, Agustin Bellosi,

Innate IL-13–producing nuocytes arise during allergic lung inflammation and contribute to airways hyperreactivity  Jillian L. Barlow, PhD, Agustin Bellosi,
Receptor for advanced glycation end products and its ligand high-mobility group box-1 mediate allergic airway sensitization and airway inflammation  Md.

Receptor for advanced glycation end products and its ligand high-mobility group box-1 mediate allergic airway sensitization and airway inflammation  Md.
Vemuri B. Reddy, PhD, Thomas A

Vemuri B. Reddy, PhD, Thomas A
Pentraxin 3 deletion aggravates allergic inflammation through a TH17-dominant phenotype and enhanced CD4 T-cell survival  Jyoti Balhara, MSc, Lianyu Shan,

Pentraxin 3 deletion aggravates allergic inflammation through a TH17-dominant phenotype and enhanced CD4 T-cell survival  Jyoti Balhara, MSc, Lianyu Shan,
IL-33 dysregulates regulatory T cells and impairs established immunologic tolerance in the lungs  Chien-Chang Chen, PhD, Takao Kobayashi, PhD, Koji Iijima,

IL-33 dysregulates regulatory T cells and impairs established immunologic tolerance in the lungs  Chien-Chang Chen, PhD, Takao Kobayashi, PhD, Koji Iijima,
Restoration of T-box–containing protein expressed in T cells protects against allergen- induced asthma  Jung Won Park, MD, Hyun Jung Min, MS, Jung Ho Sohn,

Restoration of T-box–containing protein expressed in T cells protects against allergen- induced asthma  Jung Won Park, MD, Hyun Jung Min, MS, Jung Ho Sohn,
Is 9 more than 2 also in allergic airway inflammation?

Is 9 more than 2 also in allergic airway inflammation?
Bruce M. Prenner, MD, Bobby Q. Lanier, MD, David I

Bruce M. Prenner, MD, Bobby Q. Lanier, MD, David I
Brecht Steelant, PhD, Sven F

Brecht Steelant, PhD, Sven F
Signaling through FcRγ-associated receptors on dendritic cells drives IL-33–dependent TH2-type responses  Melissa Y. Tjota, BA, Cara L. Hrusch, PhD, Kelly.

Signaling through FcRγ-associated receptors on dendritic cells drives IL-33–dependent TH2-type responses  Melissa Y. Tjota, BA, Cara L. Hrusch, PhD, Kelly.

Similar presentations

Ads by Google