1. 3a) Load or Verify IonSource & Tune File
1) Dilute Sample
2) Click
Green Button
4) Change Run time, Start mass,
End Mass, Scan time=1000
5) Click PLAY
Choose User, Sample Group, Data filename, SSI Pump checked
3b) Connect longer red peek tubing at rheodyne to solvent pump. Click TURN ON to run solvent
7) Wipe flat Syringe Outside/ Clean Inside
6) Check Stable Solvent Flow
8) Put 10-20uL diluted Sample
In LOAD
Turn to INJECT to see in MS
9) Verify TIC peak  Click Data Express 
Extract
& Label
10) STOP  Standby  Clean Syringe
& “Load Port”
3a) Load or Verify IonSource &
Tune File