Presentation is loading. Please wait.

Presentation is loading. Please wait.

Α(1,3) Fucosyltransferases IV and VII Are Essential for the Initial Recruitment of Basophils in Chronic Allergic Inflammation  Kazumi Saeki, Takahiro.

Published byDeddy Pranata Modified over 6 years ago

Similar presentations

Presentation on theme: "Α(1,3) Fucosyltransferases IV and VII Are Essential for the Initial Recruitment of Basophils in Chronic Allergic Inflammation  Kazumi Saeki, Takahiro."— Presentation transcript:

1 α(1,3) Fucosyltransferases IV and VII Are Essential for the Initial Recruitment of Basophils in Chronic Allergic Inflammation  Kazumi Saeki, Takahiro Satoh, Hiroo Yokozeki  Journal of Investigative Dermatology  Volume 133, Issue 9, Pages (September 2013) DOI: /jid Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

2 Figure 1 IgE-mediated chronic allergic inflammation (IgE-CAI) in α(1, 3) fucosyltransferase-IV (FT-IV)- and/or FT-VII-deficient mice. (a) IgE-CAI was induced in mice lacking FT-IV and/or FT-VII. Negative control mice were challenged with trinitrophenyl-OVA (TNP-OVA) without TNP-IgE injection. (b) Histopathological features of the skin (Giemsa’s staining). (c) Cell populations in inflammatory skin. (d) Basophil numbers in inflammatory skin. Basophils were detected by mouse mast cell protease-8 mAb (arrows in the right panel). *P<0.05 compared with wild-type (WT) mice. BM, basement membrane. Bar=100μm. Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

3 Figure 2 Delayed-type hypersensitivity (DTH) reactions in α(1, 3) fucosyltransferase-IV (FT-IV)- and/or FT-VII-deficient mice. Contact hypersensitivity (CHS) and DTH to sheep red blood cells (DTH-SRBCs) were induced in mice lacking FT-IV and/or -VII. *P<0.05 compared with wild-type (WT) mice. Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

4 Figure 3 α(1, 3) Fucosyltransferase-IV/VII (FT-IV/VII) in basophils are indispensable for IgE-mediated chronic allergic inflammation (IgE-CAI). IgE-CAI was induced in FcRγ(-/-) mice that received primary basophils from wild-type (WT), FT-IV(-/-), FT-VII(-/-), and FT-IV(-/-)/FT-VII(-/-) mice. (a) Although basophils from FT-IV(-/-) and FT-VII(-/-) mice induced exacerbated IgE-CAI as compared with WT basophils, (b) FT-IV(-/-)/FT-VII(-/-) mice–derived basophils were incapable of inducing IgE-CAI. *P<0.05. Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

5 Figure 4 Selectin-dependent cooperative recruitment of basophils and effector cells. (a) Irradiated α(1, 3) fucosyltransferase-IV (FT-IV)(-/-)/FT-VII(-/-) mice received wild-type (WT) basophils in combination with CD49b(-) bone marrow cells (effector cells) from either WT or FT-IV(-/-)/FT-VII(-/-) mice. They were then immunized with trinitrophenyl-IgE (TNP-IgE) and challenged with TNP-OVA. The untreated group comprised FT-IV(-/-)/FT-VII(-/-) mice without cell transfer. (b) Cell populations in inflammatory skin. *P<0.05 compared with WT effector cells. BM, basement membrane. Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

6 Figure 5 Expression of α(1, 3) fucosyltransferase (FT) messenger RNA (mRNA) and FT-dependent selectin binding in basophils. (a) Primary basophils were subjected to further purification by positive selection with CD123 (purity >99%). Transcripts for FT-IV and FT-VII mRNA were quantified by real-time PCR. (b) Binding of soluble P- and E-selectins to primary basophils assessed by flow cytometry. BMBa, bone marrow–derived basophil; BMMCs, bone marrow–derived mast cells. Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

7 Figure 6 Basophil P-selectin glycoprotein-1 (PSGL-1)–L-selectin interaction involvement in IgE-mediated chronic allergic inflammation (IgE-CAI). (a) PSGL-1 and L-selectin expressions on basophils. (b) PSGL-1 Ab effect on selectin binding to primary basophils. (c) L-selectin binding to basophils from wild-type (WT) and α(1, 3) fucosyltransferase-IV (FT-IV)(-/-)/FT-VII(-/-) mice. (d) Effects of L-selectin and/or PSGL-1 Abs on IgE-CAI in WT mice. *P<0.05 compared with control IgG. **P<0.05 compared with L-selectin or PSGL-1 Ab alone. Journal of Investigative Dermatology  , DOI: ( /jid ) Copyright © 2013 The Society for Investigative Dermatology, Inc Terms and Conditions

Download ppt "Α(1,3) Fucosyltransferases IV and VII Are Essential for the Initial Recruitment of Basophils in Chronic Allergic Inflammation  Kazumi Saeki, Takahiro."

Similar presentations

Pharmacologic Inhibition of IκB Kinase Activates Immediate Hypersensitivity Reactions in Mice  Dai Miyazaki, Sachiko Mihara, Koudai Inata, Shin-ichi Sasaki,

Pharmacologic Inhibition of IκB Kinase Activates Immediate Hypersensitivity Reactions in Mice  Dai Miyazaki, Sachiko Mihara, Koudai Inata, Shin-ichi Sasaki,
IL-17A as an Inducer for Th2 Immune Responses in Murine Atopic Dermatitis Models  Saeko Nakajima, Akihiko Kitoh, Gyohei Egawa, Yohei Natsuaki, Satoshi.

IL-17A as an Inducer for Th2 Immune Responses in Murine Atopic Dermatitis Models  Saeko Nakajima, Akihiko Kitoh, Gyohei Egawa, Yohei Natsuaki, Satoshi.
The Tumor Necrosis Factor Superfamily Molecule LIGHT Promotes Keratinocyte Activity and Skin Fibrosis  Rana Herro, Ricardo Da S. Antunes, Amelia R. Aguilera,

The Tumor Necrosis Factor Superfamily Molecule LIGHT Promotes Keratinocyte Activity and Skin Fibrosis  Rana Herro, Ricardo Da S. Antunes, Amelia R. Aguilera,
Complex 2B4 Regulation of Mast Cells and Eosinophils in Murine Allergic Inflammation  Moran Elishmereni, Nanna Fyhrquist, Roopesh Singh Gangwar, Sari Lehtimäki,

Complex 2B4 Regulation of Mast Cells and Eosinophils in Murine Allergic Inflammation  Moran Elishmereni, Nanna Fyhrquist, Roopesh Singh Gangwar, Sari Lehtimäki,
Therapeutic Potential of B and T Lymphocyte Attenuator Expressed on CD8+ T Cells for Contact Hypersensitivity  Daiki Nakagomi, Kotaro Suzuki, Junichi.

Therapeutic Potential of B and T Lymphocyte Attenuator Expressed on CD8+ T Cells for Contact Hypersensitivity  Daiki Nakagomi, Kotaro Suzuki, Junichi.
Loss of Extracellular Superoxide Dismutase Induces Severe IL-23-Mediated Skin Inflammation in Mice  Yun Sang Lee, In-Su Cheon, Byung-Hak Kim, Myung-Ja.

Loss of Extracellular Superoxide Dismutase Induces Severe IL-23-Mediated Skin Inflammation in Mice  Yun Sang Lee, In-Su Cheon, Byung-Hak Kim, Myung-Ja.
TSLP Induces Mast Cell Development and Aggravates Allergic Reactions through the Activation of MDM2 and STAT6  Na-Ra Han, Hyun-A Oh, Sun-Young Nam, Phil-Dong.

TSLP Induces Mast Cell Development and Aggravates Allergic Reactions through the Activation of MDM2 and STAT6  Na-Ra Han, Hyun-A Oh, Sun-Young Nam, Phil-Dong.
Type I IL-1 Receptor Mediates IL-1 and Intracellular IL-1 Receptor Antagonist Effects in Skin Inflammation  Gaby Palmer, Dominique Talabot-Ayer, Gürkan.

Type I IL-1 Receptor Mediates IL-1 and Intracellular IL-1 Receptor Antagonist Effects in Skin Inflammation  Gaby Palmer, Dominique Talabot-Ayer, Gürkan.
Hyper-Inflammation and Skin Destruction Mediated by Rosiglitazone Activation of Macrophages in IL-6 Deficiency  Lopa M. Das, Julie Rosenjack, Liemin Au,

Hyper-Inflammation and Skin Destruction Mediated by Rosiglitazone Activation of Macrophages in IL-6 Deficiency  Lopa M. Das, Julie Rosenjack, Liemin Au,
Yasuyo Sano, Jin Mo Park  Journal of Investigative Dermatology 

Yasuyo Sano, Jin Mo Park  Journal of Investigative Dermatology 
IL-6 Blockade Attenuates the Development of Murine Sclerodermatous Chronic Graft- Versus-Host Disease  Doanh Le Huu, Takashi Matsushita, Guihua Jin, Yasuhito.

IL-6 Blockade Attenuates the Development of Murine Sclerodermatous Chronic Graft- Versus-Host Disease  Doanh Le Huu, Takashi Matsushita, Guihua Jin, Yasuhito.
Volume 33, Issue 3, Pages (September 2010)

Volume 33, Issue 3, Pages (September 2010)
IL-17A as an Inducer for Th2 Immune Responses in Murine Atopic Dermatitis Models  Saeko Nakajima, Akihiko Kitoh, Gyohei Egawa, Yohei Natsuaki, Satoshi.

IL-17A as an Inducer for Th2 Immune Responses in Murine Atopic Dermatitis Models  Saeko Nakajima, Akihiko Kitoh, Gyohei Egawa, Yohei Natsuaki, Satoshi.
Smad4 Loss in Mouse Keratinocytes Leads to Increased Susceptibility to UV Carcinogenesis with Reduced Ercc1-Mediated DNA Repair  Doyel Mitra, Pamela Fernandez,

Smad4 Loss in Mouse Keratinocytes Leads to Increased Susceptibility to UV Carcinogenesis with Reduced Ercc1-Mediated DNA Repair  Doyel Mitra, Pamela Fernandez,
IL-27 Activates Th1-Mediated Responses in Imiquimod-Induced Psoriasis-Like Skin Lesions  Sayaka Shibata, Yayoi Tada, Yoshihide Asano, Koichi Yanaba, Makoto.

IL-27 Activates Th1-Mediated Responses in Imiquimod-Induced Psoriasis-Like Skin Lesions  Sayaka Shibata, Yayoi Tada, Yoshihide Asano, Koichi Yanaba, Makoto.
Α-MSH-Stimulated Tolerogenic Dendritic Cells Induce Functional Regulatory T Cells and Ameliorate Ongoing Skin Inflammation  Matteo Auriemma, Thomas Brzoska,

Α-MSH-Stimulated Tolerogenic Dendritic Cells Induce Functional Regulatory T Cells and Ameliorate Ongoing Skin Inflammation  Matteo Auriemma, Thomas Brzoska,
Functional Beta2-Integrins Restrict Skin Inflammation In Vivo

Functional Beta2-Integrins Restrict Skin Inflammation In Vivo
Histamine Contributes to Tissue Remodeling via Periostin Expression

Histamine Contributes to Tissue Remodeling via Periostin Expression
Enrichment for Living Murine Keratinocytes from the Hair Follicle Bulge with the Cell Surface Marker CD34  Rebecca J. Morris, Carl D. Bortner, George.

Enrichment for Living Murine Keratinocytes from the Hair Follicle Bulge with the Cell Surface Marker CD34  Rebecca J. Morris, Carl D. Bortner, George.
IL-1R1 Signaling Facilitates Munro’s Microabscess Formation in Psoriasiform Imiquimod-Induced Skin Inflammation  Mireia Uribe-Herranz, Li-Hua Lian, Kirsten.

IL-1R1 Signaling Facilitates Munro’s Microabscess Formation in Psoriasiform Imiquimod-Induced Skin Inflammation  Mireia Uribe-Herranz, Li-Hua Lian, Kirsten.

Similar presentations

Ads by Google