Presentation is loading. Please wait.

Presentation is loading. Please wait.

Immunolocalisation and expression of keratocan in tendon

Published byDoddy Sanjaya Modified over 6 years ago

Similar presentations

Presentation on theme: "Immunolocalisation and expression of keratocan in tendon"— Presentation transcript:

1 Immunolocalisation and expression of keratocan in tendon
S.G. Rees, Ph.D., A.D. Waggett, Ph.D., B.C. Kerr, Ph.D., J. Probert, B.Sc., E.C. Gealy, Ph.D., C.M. Dent, M.D., B. Caterson, Ph.D., C.E. Hughes, Ph.D.  Osteoarthritis and Cartilage  Volume 17, Issue 2, Pages (February 2009) DOI: /j.joca Copyright © 2008 Osteoarthritis Research Society International Terms and Conditions

2 Fig. 1 Immunolocalisation of keratocan in mature bovine deep digital flexor tendon. (A) Transverse section of compressed tendon immunolabelled with mAb KER-1 (shown in red) which recognises the keratocan core protein and polyclonal antibody 70-XR90 (shown in green) which recognises type I collagen. Areas of co-localisation of keratocan/collagen I immunolabelling are shown in yellow. Cell nuclei are shown in blue. Positive immunolabelling of tendon collagen fibre bundles (solid arrows) and endotenon regions (dashed arrows) is indicated. (B) Transverse sections of compressed tendon (I, II), tensional tendon (III, IV) and cornea (V), immunolabelled with mAb KER-1, and counterstained with DAPI. Images are presented as red/green anaglyphs where positive immunolabelling is shown in green and cell nuclei in red (areas of co-localisation of keratocan immunolabelling with tendon cells are shown in yellow). Positive immunolabelling of tendon collagen fibre bundles (solid arrows) and endotenon regions (dashed arrows) is indicated (I–IV). Tendon sections with the primary antibody omitted showed no immunolabelling (VI). Osteoarthritis and Cartilage  , DOI: ( /j.joca ) Copyright © 2008 Osteoarthritis Research Society International Terms and Conditions

3 Fig. 2 Western blot analyses of tissue extracts of cornea, tensional tendon (T-tend), compressed tendon (C-tend) and articular cartilage (Cart), immunolocated with mAb KER-1 which recognises the keratocan core protein. Samples were digested without (A) or with (B) endo-β-galactosidase (+β-endo) to remove KS chains. Loading of samples onto the gels was standardised by loading on the basis of equivalent protein content of tissue extract (30μg). Osteoarthritis and Cartilage  , DOI: ( /j.joca ) Copyright © 2008 Osteoarthritis Research Society International Terms and Conditions

Download ppt "Immunolocalisation and expression of keratocan in tendon"

Similar presentations

Biosynthesis of the vitamin K-dependent matrix Gla protein (MGP) in chondrocytes: a fetuin–MGP protein complex is assembled in vesicles shed from normal.

Biosynthesis of the vitamin K-dependent matrix Gla protein (MGP) in chondrocytes: a fetuin–MGP protein complex is assembled in vesicles shed from normal.
High molecular weight hyaluronic acid regulates osteoclast formation by inhibiting receptor activator of NF-κB ligand through Rho kinase  W. Ariyoshi,

High molecular weight hyaluronic acid regulates osteoclast formation by inhibiting receptor activator of NF-κB ligand through Rho kinase  W. Ariyoshi,
Effects of long-term estrogen replacement therapy on articular cartilage IGFBP-2, IGFBP-3, collagen and proteoglycan levels in ovariectomized cynomolgus.

Effects of long-term estrogen replacement therapy on articular cartilage IGFBP-2, IGFBP-3, collagen and proteoglycan levels in ovariectomized cynomolgus.
High molecular weight hyaluronic acid regulates osteoclast formation by inhibiting receptor activator of NF-κB ligand through Rho kinase  W. Ariyoshi,

High molecular weight hyaluronic acid regulates osteoclast formation by inhibiting receptor activator of NF-κB ligand through Rho kinase  W. Ariyoshi,
H. J. Pulkkinen, V. Tiitu, P. Valonen, J. S. Jurvelin, M. J. Lammi, I

H. J. Pulkkinen, V. Tiitu, P. Valonen, J. S. Jurvelin, M. J. Lammi, I
L. J. Sandell, Ph. D. , X. Xing, M. D. , C. Franz, M. A. , S

L. J. Sandell, Ph. D. , X. Xing, M. D. , C. Franz, M. A. , S
C.P. Neu, T. Novak, K.F. Gilliland, P. Marshall, S. Calve 

C.P. Neu, T. Novak, K.F. Gilliland, P. Marshall, S. Calve 
Expression and cellular localization of human hyaluronidase-2 in articular chondrocytes and cultured cell lines  G. Chow, Ph.D., C.B. Knudson, Ph.D.,

Expression and cellular localization of human hyaluronidase-2 in articular chondrocytes and cultured cell lines  G. Chow, Ph.D., C.B. Knudson, Ph.D.,
Combination of ADMSCs and chondrocytes reduces hypertrophy and improves the functional properties of osteoarthritic cartilage  M.R. Ahmed, A. Mehmood,

Combination of ADMSCs and chondrocytes reduces hypertrophy and improves the functional properties of osteoarthritic cartilage  M.R. Ahmed, A. Mehmood,
Hypoxia inducible factor-1 alpha expression in human normal and osteoarthritic chondrocytes1 1 Supported by NIH/NIAMS Program Project grant (AR-39740)

Hypoxia inducible factor-1 alpha expression in human normal and osteoarthritic chondrocytes1 1 Supported by NIH/NIAMS Program Project grant (AR-39740)
Endoglin differentially regulates TGF-β-induced Smad2/3 and Smad1/5 signalling and its expression correlates with extracellular matrix production and.

Endoglin differentially regulates TGF-β-induced Smad2/3 and Smad1/5 signalling and its expression correlates with extracellular matrix production and.
Human osteoarthritis synovial fluid and joint cartilage contain both aggrecanase- and matrix metalloproteinase-generated aggrecan fragments  A. Struglics,

Human osteoarthritis synovial fluid and joint cartilage contain both aggrecanase- and matrix metalloproteinase-generated aggrecan fragments  A. Struglics,
Biosynthesis of the vitamin K-dependent matrix Gla protein (MGP) in chondrocytes: a fetuin–MGP protein complex is assembled in vesicles shed from normal.

Biosynthesis of the vitamin K-dependent matrix Gla protein (MGP) in chondrocytes: a fetuin–MGP protein complex is assembled in vesicles shed from normal.
Differential proteome analysis of normal and osteoarthritic chondrocytes reveals distortion of vimentin network in osteoarthritis  S. Lambrecht, M.Pharm.,

Differential proteome analysis of normal and osteoarthritic chondrocytes reveals distortion of vimentin network in osteoarthritis  S. Lambrecht, M.Pharm.,
Pressure distention compared with pharmacologic relaxation in vein grafting upregulates matrix metalloproteinase-2 and -9  Ada W.Y. Chung, PhD, Pooja.

Pressure distention compared with pharmacologic relaxation in vein grafting upregulates matrix metalloproteinase-2 and -9  Ada W.Y. Chung, PhD, Pooja.
Articular chondrocytes derived from distinct tissue zones differentially respond to in vitro oscillatory tensile loading  E.J. Vanderploeg, Ph.D., C.G.

Articular chondrocytes derived from distinct tissue zones differentially respond to in vitro oscillatory tensile loading  E.J. Vanderploeg, Ph.D., C.G.
Tissue engineering of cartilage using an injectable and adhesive chitosan-based cell- delivery vehicle  C.D. Hoemann, Ph.D., J. Sun, M.D., A. Légaré, M.Sc.,

Tissue engineering of cartilage using an injectable and adhesive chitosan-based cell- delivery vehicle  C.D. Hoemann, Ph.D., J. Sun, M.D., A. Légaré, M.Sc.,
Aggrecanolysis in human osteoarthritis: confocal localization and biochemical characterization of ADAMTS5–hyaluronan complexes in articular cartilages 

Aggrecanolysis in human osteoarthritis: confocal localization and biochemical characterization of ADAMTS5–hyaluronan complexes in articular cartilages 
R. Piva, E. Lambertini, C. Manferdini, C. Capanni, L. Penolazzi, E

R. Piva, E. Lambertini, C. Manferdini, C. Capanni, L. Penolazzi, E
Evidence for enhanced collagen type III deposition focally in the territorial matrix of osteoarthritic hip articular cartilage  S. Hosseininia, M.A. Weis,

Evidence for enhanced collagen type III deposition focally in the territorial matrix of osteoarthritic hip articular cartilage  S. Hosseininia, M.A. Weis,

Similar presentations

Ads by Google