1. Quantitative Chemical Analysis
Daniel C. Harris
Quantitative Chemical Analysis
Seventh Edition
Chapter 23
Chromatography
Copyright © 2007 by W. H. Freeman and Company

7. Example: V1 = 100 mL, K = 3
(1) Extract with 500 mL
And 5 times with 500 mL?
(2) Extract 5 times with 100 mL

8. How to cleverly do
multiple extractions:
Move one phase past
The other

13. Separation factor:
γ = tr2/tr1

16. So you can measure the outcome in volume or in time

17. The ideal Gaussian peak

18. resolving two Gaussian peaks

19. Can you improve resolution
by just using a longer column
(to spread out the peaks further)?
Not necessarily!

20. Therefore resolution improves
with square root of the column length

22. NUMBER OF PLATES: