1. A New Rapid Method for Clostridium difficile DNA Extraction and Detection in Stool 
Alison G. Freifeld, Kari A. Simonsen, Christine S. Booth, Xing Zhao, Scott E. Whitney, Teresa Karre, Peter C. Iwen, Hendrik J. Viljoen 
The Journal of Molecular Diagnostics 
Volume 14, Issue 3, Pages (May 2012)
DOI: /j.jmoldx
Copyright © 2012 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

2. Figure 1
The rapid LMR/PCR process. A stool sample is mixed with lysis buffer and transferred to the LMR (B) along with Bio-Rad Chelex 100 resin beads. A capture strip (A) with cap is inserted into the LMR (B), and the contents are heated and mixed. Single-stranded DNA from lysed cells binds to the strip. After lysis is complete, the strip is removed, washed, and inserted into a cuvette containing master mix (C) for rapid PCR amplification in the Philisa Thermo Cycler (Streck, Omaha, NE) (D).
The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx )
Copyright © 2012 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

3. Figure 2
Agarose gel electrophoresis results of PCR products after tcdB amplification for stool samples spiked with C. difficile DNA to concentrations of 0.5 pg/mL (which corresponds to 114 copies/mL; lane 2), 0.05 pg/mL (11 copies/mL; lane 3) and 0.01 pg/mL (2 copies/mL; lane 4). Results are shown without Chelex 100 resin added to the LMR mixture (A) or with Chelex 100 resin (B). Positive control (PC) indicates GDH+/toxin+ stool; negative control (NC) indicates GDH−/toxin− stool.
The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx )
Copyright © 2012 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions