1. Behavioral sequence.
Behavioral sequence. Behaviors were quantified during five 5-min assessments. Anxiety-related behaviors were quantified in the light/dark (L/D) box during assessment 1. Mice were placed in the dark side of the apparatus. Two minutes later, a door separating the two sides was removed, and mice were able to explore both the dark and light sides of the apparatus freely for an additional 3 min. Motor behaviors were quantified in a visually cued cylindrical arena during assessment 2. Attention and short-term memory were tested during assessments 3–5. During assessment 3 (initial exposure), mice explored two objects in positions a and b. Object recognition behavior was tested during assessment 4 (novel object) by replacing the familiar object in position b with a novel object. Location recognition was tested during assessment 5 (novel location) by moving the familiar object from position a to position c.
Aldis P. Weible et al. PNAS 2017;114:10:
©2017 by National Academy of Sciences