1. Na+-dependent fluid absorption in intact perfused rat colonic crypts
John P. Geibel, Vazhaikkurichi M. Rajendran, Henry J. Binder 
Gastroenterology 
Volume 120, Issue 1, Pages (January 2001)
DOI: /gast
Copyright © 2001 American Gastroenterological Association Terms and Conditions

2. Fig. 1
Effect of inhibition of Cl− secretion on net fluid absorption in isolated, perfused colonic crypt. Net fluid movement was determined in the presence of either 50 or 100 μmol/L bath bumetanide, an inhibitor of Na-K-2Cl cotransport. *P < 0.01, **P < 0.001; compared with control. Results are mean ± SEM. Number in parentheses represents the number of animals studied in each experiment. □, Control; ▨, 50 μmol/L bumetanide; ■, 100 μmol/L bumetanide.
Gastroenterology  , DOI: ( /gast )
Copyright © 2001 American Gastroenterological Association Terms and Conditions

3. Fig. 2
Role of lumen Na+ on fluid absorption (Jv) in isolated, perfused colonic crypt. (A) Control perfusion contained 140 mmol/L Na+, whereas in the Na+-free experiment, lumen Na+ was replaced by 140 mmol/L NMDG. *P < compared with 140 mmol/L Na+ control. (B) Effect of 1 μmol/L EIPA, an amiloride analogue that selectively inhibits Na+-H exchange, on net fluid movement. Control solution contained 50 mmol/L Na+. **P < compared with 50 mmol/L Na+ control. Results are mean ± SEM. Number in parentheses represents the number of animals studied in each experiment.
Gastroenterology  , DOI: ( /gast )
Copyright © 2001 American Gastroenterological Association Terms and Conditions

4. Fig. 3
Effect of lumen and bath Cl− on net fluid absorption in the presence or absence of bath Cl− in the isolated, perfused colonic crypt. Either lumen or bath Cl− was selectively replaced by cyclamate. *P < compared with lumen Cl−-Ringer. **P < 0.05 compared with lumen and bath Cl−-Ringer. Results are mean ± SEM. Number in parentheses represents the number of animals studied in each experiment. □, Lumen Cl−-Ringer; ▨, lumen Cl−-free Ringer.
Gastroenterology  , DOI: ( /gast )
Copyright © 2001 American Gastroenterological Association Terms and Conditions

5. Fig. 4
Effect of Cl− transport inhibitors on net fluid absorption in isolated, perfused colonic crypt. DIDS is an inhibitor of anion exchange at 100 μmol/L, but of Cl− channels at 500 μmol/L.10 NPPB is an inhibitor of Cl− channels at a dose of 10 μmol/L. *P < **P < †P < Results are mean ± SEM. Number in parentheses represents the number of animals studied in each experiment. □, Control; ▩, lumen NPPB; ▨, lumen DIDS (100 μmol/L); ▩, lumen DIDS (500 μmol/L).
Gastroenterology  , DOI: ( /gast )
Copyright © 2001 American Gastroenterological Association Terms and Conditions

6. Fig. 5
Effect of acetazolamide on net fluid absorption in isolated, perfused colonic crypt. Cl− was present in the studies shown in the left 2 histograms, but was replaced by cyclomate in both lumen and bath in the studies presented in the right 2 histograms. *P < compared with Cl−-free Ringer control. Results are mean ± SEM. Number in parentheses represents the number of animals studied in each experiment. □, Cl−-Ringer; ■, Cl−-free Ringer; ▨, acetazolamide.
Gastroenterology  , DOI: ( /gast )
Copyright © 2001 American Gastroenterological Association Terms and Conditions