1. Fluorescence activated cell sorting: A reliable method in tissue engineering of a bioprosthetic heart valve 
Simon P Hoerstrup, MD, Gregor Zünd, MD, Andreina Schoeberlein, PhD, Qing Ye, MD, Paul R Vogt, MD, Marko I Turina, MD 
The Annals of Thoracic Surgery 
Volume 66, Issue 5, Pages (November 1998)
DOI: /S (98)

2. Fig 1
Concept of tissue engineering of a bioprosthetic heart valve. (FACS = fluorescence activated cell sorting.)
The Annals of Thoracic Surgery  , DOI: ( /S (98) )

3. Fig 2
Histogram flow cytometry. (A) Negative control, unlabeled mixed cell population of human aortic tissue. (B) Positive assay, Dil-Ac-LDL–labeled mixed cell population of human aortic tissue (endothelial cells = LDL+ [fluorescent], myofibroblasts = LDL−). (C) “Gating” of significant areas for fluorescence activated cell sorting. (M3 = nonfluorescent [myofibroblasts]; M2 = fluorescent [endothelial cells]; M1 = with regard to fluorescence ambiguous cell population [safety margin for sorting procedure].)
The Annals of Thoracic Surgery  , DOI: ( /S (98) )

4. Fig 3
Fluorescence microscopy: full fluorescence of the endothelial cell line (LDL+). (×100 before 35% reduction.)
The Annals of Thoracic Surgery  , DOI: ( /S (98) )

5. Fig 4
Formation of endothelial monolayer. CD34 stain demonstrates a monolayer of endothelial cells (a) on the surface of a fibroblast core (b) with polymer fibers (c).
The Annals of Thoracic Surgery  , DOI: ( /S (98) )