1. Distribution of respiratory pathogens identified in patients using clinician-ordered diagnostics versus mNGS.
Distribution of respiratory pathogens identified in patients using clinician-ordered diagnostics versus mNGS. Number of subjects in whom each respiratory microbe was detected. All microbes detected by clinician-ordered diagnostics were detected by mNGS; however, pink bars indicate microbes misclassified as negative by either the RBM or LRM. Notably, all microbes identified by clinician-ordered diagnostics and misclassified by either the RBM or LRM (pink bars) were found in polymicrobial cultures, highlighting the presence of dominant pathogens by NGS that are not captured in the polymicrobial culture results. Red bars indicate microbes detected by clinician-ordered diagnostics and also predicted as pathogens by either the RBM or LRM. More detail on which model identified each microbe can be found in SI Appendix, Fig. S2. Dark red bars (LRTI+C+M and LRTI+C subjects) and gray bars (no-LRTI subjects) indicate number of cases with microbes detected only by mNGS.
Charles Langelier et al. PNAS doi: /pnas
©2018 by National Academy of Sciences