1. Research Techniques Made Simple: Bacterial 16S Ribosomal RNA Gene Sequencing in Cutaneous Research 
Jay-Hyun Jo, Elizabeth A. Kennedy, Heidi H. Kong 
Journal of Investigative Dermatology 
Volume 136, Issue 3, Pages e23-e27 (March 2016)
DOI: /j.jid
Copyright © 2016 The Authors Terms and Conditions

2. Figure 1
Schematic illustration of basic workflow for skin 16S rRNA gene-based sequencing.
Adapted and modified with permission from Kong (2011).
Journal of Investigative Dermatology  , e23-e27DOI: ( /j.jid )
Copyright © 2016 The Authors Terms and Conditions

3. Figure 2
16S rRNA gene structure illustrating variable (blue, V1–V9) and conserved (dark gray) regions. Common regions (amplicons) for next-generation sequencing-based microbial community profiling are depicted below.
Journal of Investigative Dermatology  , e23-e27DOI: ( /j.jid )
Copyright © 2016 The Authors Terms and Conditions

4. Figure 3
Skin microbial structure is closely correlated with host status. (a) Relative abundance (phylum and order level) for wild-type (WT) and hypomorphic matriptase (St14hypo/−) mice. Adapted from Scharschmidt et al. (2009). (b) Relative genus (upper) and Staphylococcus species (lower) abundance in the antecubital and popliteal creases for atopic dermatitis status: baseline, flare (with or without intermittent treatment), and postflare. Each bar represents individual patients.
Adapted with permission from Kong et al. (2012).
Journal of Investigative Dermatology  , e23-e27DOI: ( /j.jid )
Copyright © 2016 The Authors Terms and Conditions