1. In vitro analysis of K107R white adipocytes.
In vitro analysis of K107R white adipocytes. (A) WT and K107R stromal vascular cells were differentiated using insulin, dexamethasone, and IBMX in the presence of rosiglitazone or vehicle. RNA was prepared at the indicated times, and Cebpb, Cebpa, and Pparg expression was measured by qPCR. (B) Neutral lipid accumulation was measured by Oil Red O staining at the indicated times. (C, Left) Immunoblotting for SUMO1-conjugated PPARγ (SUMO1) and total PPARγ following PPARγ immunoprecipitation. (Right) Quantification of SUMO1-conjugated PPARγ normalized to PPARγ2. *P < 0.05; **P < 0.01; ***P <
Takeshi Katafuchi et al. PNAS 2018;115:48:
©2018 by National Academy of Sciences