1. Host transcriptional profiling distinguishes patients with acute LRTI (LRTI+C+M) from those with noninfectious acute respiratory illness (no-LRTI).
Host transcriptional profiling distinguishes patients with acute LRTI (LRTI+C+M) from those with noninfectious acute respiratory illness (no-LRTI). (A) Host classifier scores for all patients in the derivation and validation cohorts; each bar indicates a patient score and is colored as follows: LRTI+C+M, red; no-LRTI, blue. Orange dotted line indicates the host classifier threshold (score, −4) that achieved 100% sensitivity in the training set and was used to classify the test set samples. (B) Normalized expression levels, arranged by unsupervised hierarchical clustering, reflect overexpression (blue) or underexpression (turquoise) of classifier genes (rows) for each patient (columns). Twelve genes were identified as predictive in the derivation cohort and subsequently applied to predict LRTI status in the validation cohort. Column colors above the heatmap indicate whether a patient belonged to the derivation cohort (dark gray) or validation cohort (light gray) and whether they were adjudicated to have LRTI+C+M (red) or no-LRTI (blue). (C) ROC curves demonstrating host classifier performance for derivation (blue) and validation (green) cohorts.
Charles Langelier et al. PNAS doi: /pnas
©2018 by National Academy of Sciences