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Aim: Microscope Parts … YEAH!!
Enter Date Aim: Microscope Parts … YEAH!! Warm-up: Write a hypothesis for the statement … Chocolate may cause pimples HW:
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Microscope Terms Magnification How LARGE an image is Resolution
How sharp or clear the image is Fine adjustment aids in this
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Base Body Tube Nosepiece Eye Piece Arm Low Power Stage High Power
Stage Clips Coarse Adjustment Diaphragm Fine Adjustment Light Source Base
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Compound-light Microscope
2 sets of lenses eyepiece & objective Specimens must be thin & often dead Image is upside-down & backwards e ə
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Moving Objective Under the Microscope
To center objects you need to realize that everything is REVERSED NEED to move it to the LEFT
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In which direction should the slide be moved to center the cell?
To the right & up 2) To the right and down 3) To the left and up 4) To the left and down
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Cheek Cells Red Blood Cells with Cholesterol
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Paramecium Pollen Grains
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Clockwise from top left: an ant, passion fruit pollen, a freshwater shrimp parasite, sunflower pollen and central, a green bottle foot.
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Microscope Measurement
1. Total Magnification Eyepiece x Objective 100X Ex: 1: 10 x 10 = Ex. 2: 10 x 43 = 430X
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2. Micrometer/Micron (μm)
Unit of measurement used with the microscope 1 mm = 1000 um
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mm um Move decimal 3 places to right or Multiply by 1000 5,000 um
Ex. 1: 5 mm = ? um Ex. 2: 1.5 mm = ? um 1,500 um
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um mm Move decimal 3 places to left or Divide by 1000 0.008 mm
Ex. 1: 8 um = ? mm Ex. 2: 2.5 um = ? mm mm
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About how many um are each cell?
2mm = 2000 um = 400 um/cell 5 cells
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What is the approximate diameter of the cell shown in the low-power field?
100 um 2) 500 um 3) 800 um 4) 1,000 um 1000 um 1mm = 1000 um = 500 um/cell 2 cells
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3. Changing from Power to Power
Low Power Bigger field of view See more cells, but in less detail Brighter High Power Smaller field of view See less cells, but in more detail Darker
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Low Power: 40X
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High Power: 400X
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Low Power: 40X
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High Power: 400X
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